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Assay Detail

CHEMBL2091389

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

E3 ubiquitin-protein ligase Mdm2 (CHEMBL5023)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
Guo Z, Zhuang C, Zhu L, Zhang Y, Yao J, Dong G, Wang S, Liu Y, Chen H, Sheng C, Miao Z, Zhang W.
Eur J Med Chem (2012) 56 : 10 -16
PubMed 22940704 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 22 5.82 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2089208 1 7.05
CHEMBL2089203 1 7.04
CHEMBL191334 NUTLIN-3 1 6.92
CHEMBL2089201 1 6.50
CHEMBL2089211 1 6.29
CHEMBL2089152 1 6.15
CHEMBL2089151 1 6.14
CHEMBL2089209 1 5.98
CHEMBL2089149 1 5.86
CHEMBL2089200 1 5.76
CHEMBL2089204 1 5.52
CHEMBL2089210 1 5.40
CHEMBL1909836 1 5.17
CHEMBL2089202 1 5.09
CHEMBL2089320 1 4.92
CHEMBL2089322 1 4.68
CHEMBL2089207 1 4.42
CHEMBL2089206 1 -
CHEMBL2089321 1 -
CHEMBL2089205 1 -
CHEMBL2089153 1 -
CHEMBL2089150 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2089208 Ki = 89.0 nM 7.05
CHEMBL2089203 Ki = 91.0 nM 7.04
CHEMBL191334 NUTLIN-3 Ki = 121.0 nM 6.92
CHEMBL2089201 Ki = 315.0 nM 6.50
CHEMBL2089211 Ki = 518.0 nM 6.29
CHEMBL2089152 Ki = 707.0 nM 6.15
CHEMBL2089151 Ki = 721.0 nM 6.14
CHEMBL2089209 Ki = 1050.0 nM 5.98
CHEMBL2089149 Ki = 1370.0 nM 5.86
CHEMBL2089200 Ki = 1720.0 nM 5.76
CHEMBL2089204 Ki = 3020.0 nM 5.52
CHEMBL2089210 Ki = 3980.0 nM 5.40
CHEMBL1909836 Ki = 6700.0 nM 5.17
CHEMBL2089202 Ki = 8180.0 nM 5.09
CHEMBL2089320 Ki = 11900.0 nM 4.92
CHEMBL2089322 Ki = 21100.0 nM 4.68
CHEMBL2089207 Ki = 37700.0 nM 4.42
CHEMBL2089206 Ki > 100000.0 nM -
CHEMBL2089321 Ki > 100000.0 nM -
CHEMBL2089205 Ki > 100000.0 nM -
CHEMBL2089153 Ki > 100000.0 nM -
CHEMBL2089150 Ki > 100000.0 nM -