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Assay Detail

CHEMBL2092007

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MEK1-mediated ERK1 phosphorylation after 30 mins by HTRF assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Publication

Discovery of novel allosteric mitogen-activated protein kinase kinase (MEK) 1,2 inhibitors possessing bidentate Ser212 interactions.
Heald RA, Jackson P, Savy P, Jones M, Gancia E, Burton B, Newman R, Boggs J, Chan E, Chan J, Choo E, Merchant M, Rudewicz P, Ultsch M, Wiesmann C, Yue Q, Belvin M, Price S.
J Med Chem (2012) 55 : 4594 -4604
PubMed 22506516 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.27 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2087076 1 7.89
CHEMBL2087080 1 7.82
CHEMBL2087461 1 7.82
CHEMBL2087462 1 7.70
CHEMBL2087077 1 7.60
CHEMBL2087078 1 7.46
CHEMBL2087081 1 7.10
CHEMBL2087082 1 7.03
CHEMBL2087079 1 6.90
CHEMBL2087460 1 5.34
CHEMBL2087083 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2087076 IC50 = 13.0 nM 7.89
CHEMBL2087080 IC50 = 15.0 nM 7.82
CHEMBL2087461 IC50 = 15.0 nM 7.82
CHEMBL2087462 IC50 = 20.0 nM 7.70
CHEMBL2087077 IC50 = 25.0 nM 7.60
CHEMBL2087078 IC50 = 35.0 nM 7.46
CHEMBL2087081 IC50 = 79.0 nM 7.10
CHEMBL2087082 IC50 = 93.0 nM 7.03
CHEMBL2087079 IC50 = 125.0 nM 6.90
CHEMBL2087460 IC50 = 4546.0 nM 5.34
CHEMBL2087083 IC50 > 10000.0 nM -