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Assay Detail

CHEMBL2092208

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human His6-tagged recombinant FPPS expressed in Escherichia coli BL21(DE3) using GPP and [3H]IPP as substrate incubated for 5 mins prior to substrate addition measured after 20 mins by liquid scintillation counting
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Farnesyl pyrophosphate synthase (CHEMBL1782)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design of potent bisphosphonate inhibitors of the human farnesyl pyrophosphate synthase via targeted interactions with the active site 'capping' phenyls.
De Schutter JW, Shaw J, Lin YS, Tsantrizos YS.
Bioorg Med Chem (2012) 20 : 5583 -5591
PubMed 22884353 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.12 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL923 RISEDRONIC ACID 4.0 1 7.96
CHEMBL2088336 1 7.92
CHEMBL2088339 1 7.80
CHEMBL2048241 1 7.55
CHEMBL2048238 1 7.33
CHEMBL2088338 1 7.22
CHEMBL291736 1 7.07
CHEMBL2048247 1 6.94
CHEMBL2048239 1 6.82
CHEMBL2048237 1 6.79
CHEMBL2088337 1 6.00
CHEMBL2088344 1 6.00
CHEMBL2088342 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL923 RISEDRONIC ACID IC50 = 11.0 nM 7.96
CHEMBL2088336 IC50 = 12.0 nM 7.92
CHEMBL2088339 IC50 = 16.0 nM 7.80
CHEMBL2048241 IC50 = 28.0 nM 7.55
CHEMBL2048238 IC50 = 47.0 nM 7.33
CHEMBL2088338 IC50 = 60.0 nM 7.22
CHEMBL291736 IC50 = 85.0 nM 7.07
CHEMBL2048247 IC50 = 115.0 nM 6.94
CHEMBL2048239 IC50 = 150.0 nM 6.82
CHEMBL2048237 IC50 = 164.0 nM 6.79
CHEMBL2088337 IC50 = 1000.0 nM 6.00
CHEMBL2088344 IC50 = 1000.0 nM 6.00
CHEMBL2088342 IC50 > 1000.0 nM -