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Assay Detail

CHEMBL2149801

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of DPP4 in rat plasma using GLY-Pro-MCA as substrate after 60 mins by fluorescence assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Plasma
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dipeptidyl peptidase 4 (CHEMBL4653)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery and preclinical profile of teneligliptin (3-[(2S,4S)-4-[4-(3-methyl-1-phenyl-1H-pyrazol-5-yl)piperazin-1-yl]pyrrolidin-2-ylcarbonyl]thiazolidine): a highly potent, selective, long-lasting and orally active dipeptidyl peptidase IV inhibitor for the treatment of type 2 diabetes.
Yoshida T, Akahoshi F, Sakashita H, Kitajima H, Nakamura M, Sonda S, Takeuchi M, Tanaka Y, Ueda N, Sekiguchi S, Ishige T, Shima K, Nabeno M, Abe Y, Anabuki J, Soejima A, Yoshida K, Takashina Y, Ishii S, Kiuchi S, Fukuda S, Tsutsumiuchi R, Kosaka K, Murozono T, Nakamaru Y, Utsumi H, Masutomi N, Kishida H, Miyaguchi I, Hayashi Y.
Bioorg Med Chem (2012) 20 : 5705 -5719
PubMed 22959556 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 8.83 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2147704 1 9.74
CHEMBL2147703 1 9.72
CHEMBL2147777 TENELIGLIPTIN 4.0 1 9.54
CHEMBL2147709 1 9.51
CHEMBL2147712 1 9.31
CHEMBL2147713 1 9.28
CHEMBL2147707 1 9.16
CHEMBL2147771 1 9.14
CHEMBL2147773 1 9.11
CHEMBL2058969 1 9.00
CHEMBL2147711 1 8.64
CHEMBL2147710 1 8.60
CHEMBL2147705 1 8.54
CHEMBL2147714 1 8.54
CHEMBL2058968 1 8.52
CHEMBL2147706 1 8.51
CHEMBL2147772 1 8.33
CHEMBL2147702 1 8.27
CHEMBL2147775 1 8.24
CHEMBL2147715 1 7.89
CHEMBL2147774 1 7.85
CHEMBL2147776 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2147704 IC50 = 0.18 nM 9.74
CHEMBL2147703 IC50 = 0.19 nM 9.72
CHEMBL2147777 TENELIGLIPTIN IC50 = 0.29 nM 9.54
CHEMBL2147709 IC50 = 0.31 nM 9.51
CHEMBL2147712 IC50 = 0.49 nM 9.31
CHEMBL2147713 IC50 = 0.53 nM 9.28
CHEMBL2147707 IC50 = 0.69 nM 9.16
CHEMBL2147771 IC50 = 0.72 nM 9.14
CHEMBL2147773 IC50 = 0.77 nM 9.11
CHEMBL2058969 IC50 = 1.0 nM 9.00
CHEMBL2147711 IC50 = 2.3 nM 8.64
CHEMBL2147710 IC50 = 2.5 nM 8.60
CHEMBL2147705 IC50 = 2.9 nM 8.54
CHEMBL2147714 IC50 = 2.9 nM 8.54
CHEMBL2058968 IC50 = 3.0 nM 8.52
CHEMBL2147706 IC50 = 3.1 nM 8.51
CHEMBL2147772 IC50 = 4.7 nM 8.33
CHEMBL2147702 IC50 = 5.4 nM 8.27
CHEMBL2147775 IC50 = 5.7 nM 8.24
CHEMBL2147715 IC50 = 13.0 nM 7.89
CHEMBL2147774 IC50 = 14.0 nM 7.85
CHEMBL2147776 IC50 > 1000.0 nM -