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Assay Detail

CHEMBL2154079

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of COX2-mediated PGE2 production in LPS-induced mouse J774 cells by radioimmunoassay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type J774
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin G/H synthase 2 (CHEMBL4321)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Improving the solubility of a new class of antiinflammatory pharmacodynamic hybrids, that release nitric oxide and inhibit cycloxygenase-2 isoenzyme.
Biava M, Battilocchio C, Poce G, Alfonso S, Consalvi S, Porretta GC, Schenone S, Calderone V, Martelli A, Testai L, Ghelardini C, Di Cesare Mannelli L, Sautebin L, Rossi A, Giordani A, Patrignani P, Anzini M.
Eur J Med Chem (2012) 58 : 287 -298
PubMed 23131542 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.04 6.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2153831 1 6.66
CHEMBL2153829 1 6.12
CHEMBL2153825 1 6.09
CHEMBL2153827 1 6.00
CHEMBL2153830 1 5.92
CHEMBL2153828 1 5.85
CHEMBL2153826 1 5.82
CHEMBL2153832 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2153831 IC50 = 220.0 nM 6.66
CHEMBL2153829 IC50 = 760.0 nM 6.12
CHEMBL2153825 IC50 = 820.0 nM 6.09
CHEMBL2153827 IC50 = 1000.0 nM 6.00
CHEMBL2153830 IC50 = 1190.0 nM 5.92
CHEMBL2153828 IC50 = 1410.0 nM 5.85
CHEMBL2153826 IC50 = 1510.0 nM 5.82
CHEMBL2153832 IC50 = 1500.0 nM 5.82