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Assay Detail

CHEMBL2155148

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of p38MAPKalpha using GST-ATF2 (1 to 115) as substrate preincubated for 30 mins before [gamma-32P]-ATP addition measured after 10 mins by liquid scintillation counting in presence of 100 units/mL of catalase
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 14 (CHEMBL260)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

From in Silico Discovery to intra-Cellular Activity: Targeting JNK-Protein Interactions with Small Molecules.
Kaoud TS, Yan C, Mitra S, Tseng CC, Jose J, Taliaferro JM, Tuohetahuntila M, Devkota A, Sammons R, Park J, Park H, Shi Y, Hong J, Ren P, Dalby KN.
ACS Med Chem Lett (2012) 3 : 721 -725
PubMed 23002419 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.30 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2151190 1 6.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2151190 IC50 = 500.0 nM 6.30