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Assay Detail

CHEMBL2156338

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant GCP2 using N-acetyl-L-aspartyl-[3H]-L-glutamate as substrate by microplate assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glutamate carboxypeptidase 2 (CHEMBL1892)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and pharmacological evaluation of glutamate carboxypeptidase II (GCPII) inhibitors based on thioalkylbenzoic acid scaffolds.
Stoermer D, Vitharana D, Hin N, Delahanty G, Duvall B, Ferraris DV, Grella BS, Hoover R, Rojas C, Shanholtz MK, Smith KP, Stathis M, Wu Y, Wozniak KM, Slusher BS, Tsukamoto T.
J Med Chem (2012) 55 : 5922 -5932
PubMed 22642259 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.99 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2152556 1 8.70
CHEMBL2152561 1 8.70
CHEMBL2152562 1 8.15
CHEMBL382398 1 7.82
CHEMBL2152557 1 7.80
CHEMBL1287900 1 7.66
CHEMBL2152437 1 7.42
CHEMBL2152555 1 7.39
CHEMBL2152438 1 7.28
CHEMBL2152565 1 7.16
CHEMBL303352 1 7.05
CHEMBL2152564 1 6.85
CHEMBL2152566 1 6.70
CHEMBL2152563 1 6.70
CHEMBL2152560 1 6.64
CHEMBL2152436 1 6.47
CHEMBL2152439 1 6.38
CHEMBL2152558 1 6.03
CHEMBL2152434 1 5.89
CHEMBL2152559 1 5.85
CHEMBL294565 1 5.85
CHEMBL2152435 1 5.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2152556 IC50 = 2.0 nM 8.70
CHEMBL2152561 IC50 = 2.0 nM 8.70
CHEMBL2152562 IC50 = 7.0 nM 8.15
CHEMBL382398 IC50 = 15.0 nM 7.82
CHEMBL2152557 IC50 = 16.0 nM 7.80
CHEMBL1287900 IC50 = 22.0 nM 7.66
CHEMBL2152437 IC50 = 38.0 nM 7.42
CHEMBL2152555 IC50 = 41.0 nM 7.39
CHEMBL2152438 IC50 = 52.0 nM 7.28
CHEMBL2152565 IC50 = 69.0 nM 7.16
CHEMBL303352 IC50 = 90.0 nM 7.05
CHEMBL2152564 IC50 = 140.0 nM 6.85
CHEMBL2152566 IC50 = 200.0 nM 6.70
CHEMBL2152563 IC50 = 200.0 nM 6.70
CHEMBL2152560 IC50 = 230.0 nM 6.64
CHEMBL2152436 IC50 = 340.0 nM 6.47
CHEMBL2152439 IC50 = 420.0 nM 6.38
CHEMBL2152558 IC50 = 930.0 nM 6.03
CHEMBL2152434 IC50 = 1300.0 nM 5.89
CHEMBL2152559 IC50 = 1400.0 nM 5.85
CHEMBL294565 IC50 = 1400.0 nM 5.85
CHEMBL2152435 IC50 = 5700.0 nM 5.24