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Assay Detail

CHEMBL2157129

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]LSD from human 5HT6 receptor expressed in HEK293 cells after 1.5 hrs by liquid scintillation counting
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 6 (CHEMBL3371)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemocentric informatics approach to drug discovery: identification and experimental validation of selective estrogen receptor modulators as ligands of 5-hydroxytryptamine-6 receptors and as potential cognition enhancers.
Hajjo R, Setola V, Roth BL, Tropsha A.
J Med Chem (2012) 55 : 5704 -5719
PubMed 22537153 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 6.43 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL42 CLOZAPINE 4.0 1 7.77
CHEMBL1628227 DOXEPIN 4.0 1 6.98
CHEMBL415 CLOMIPRAMINE 4.0 1 6.95
CHEMBL53904 ZUCLOPENTHIXOL 3.0 1 6.77
CHEMBL445 NORTRIPTYLINE 4.0 1 6.67
CHEMBL81 RALOXIFENE 4.0 1 6.12
CHEMBL83 TAMOXIFEN 4.0 1 5.98
CHEMBL46516 FLUSPIRILENE 3.0 1 5.92
CHEMBL2355051 CLOMIPHENE 4.0 1 5.71
CHEMBL1655 TOREMIFENE 4.0 1 5.38
CHEMBL254832 FENDILINE 2.0 1 -
CHEMBL98350 LY-294002 -1.0 1 -
CHEMBL25236 BP-897 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL42 CLOZAPINE Ki = 17.0 nM 7.77
CHEMBL1628227 DOXEPIN Ki = 105.0 nM 6.98
CHEMBL415 CLOMIPRAMINE Ki = 112.0 nM 6.95
CHEMBL53904 ZUCLOPENTHIXOL Ki = 169.0 nM 6.77
CHEMBL445 NORTRIPTYLINE Ki = 214.0 nM 6.67
CHEMBL81 RALOXIFENE Ki = 750.0 nM 6.12
CHEMBL83 TAMOXIFEN Ki = 1041.0 nM 5.98
CHEMBL46516 FLUSPIRILENE Ki = 1188.0 nM 5.92
CHEMBL2355051 CLOMIPHENE Ki = 1956.0 nM 5.71
CHEMBL1655 TOREMIFENE Ki = 4125.0 nM 5.38
CHEMBL254832 FENDILINE Ki - -
CHEMBL98350 LY-294002 Ki - -
CHEMBL25236 BP-897 Ki - -