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Assay Detail

CHEMBL2161050

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human MAGL assessed as [3H]-2-OG hydrolysis preincubated for 30 mins before [3H]-2-OG addition measured after 10 mins by liquid scintillation counting
38
Total Activities
38
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Monoglyceride lipase (CHEMBL4191)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and characterization of endocannabinoid hydrolases FAAH and MAGL inhibitors bearing a benzotriazol-1-yl carboxamide scaffold.
Morera L, Labar G, Ortar G, Lambert DM.
Bioorg Med Chem (2012) 20 : 6260 -6275
PubMed 23036333 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 38 7.45 9.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2159781 1 9.27
CHEMBL2159805 1 8.70
CHEMBL2159780 1 8.52
CHEMBL2159800 1 8.52
CHEMBL2159812 1 8.15
CHEMBL2159798 1 8.10
CHEMBL2159806 1 8.10
CHEMBL2159803 1 8.05
CHEMBL2159779 1 8.05
CHEMBL2159801 1 8.00
CHEMBL2159802 1 7.96
CHEMBL2159804 1 7.92
CHEMBL2159811 1 7.89
CHEMBL2159795 1 7.72
CHEMBL2159788 1 7.70
CHEMBL2159797 1 7.68
CHEMBL2159777 1 7.64
CHEMBL2159782 1 7.52
CHEMBL2159787 1 7.50
CHEMBL2159778 1 7.48
CHEMBL2159799 1 7.48
CHEMBL2159809 1 7.44
CHEMBL576786 1 7.43
CHEMBL2159790 1 7.42
CHEMBL2159794 1 7.39
CHEMBL2159813 1 7.21
CHEMBL2159789 1 7.20
CHEMBL2159783 1 7.15
CHEMBL2159808 1 6.99
CHEMBL2159792 1 6.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2159781 IC50 = 0.54 nM 9.27
CHEMBL2159805 IC50 = 2.0 nM 8.70
CHEMBL2159800 IC50 = 3.0 nM 8.52
CHEMBL2159780 IC50 = 3.0 nM 8.52
CHEMBL2159812 IC50 = 7.0 nM 8.15
CHEMBL2159798 IC50 = 8.0 nM 8.10
CHEMBL2159806 IC50 = 8.0 nM 8.10
CHEMBL2159803 IC50 = 9.0 nM 8.05
CHEMBL2159779 IC50 = 9.0 nM 8.05
CHEMBL2159801 IC50 = 10.0 nM 8.00
CHEMBL2159802 IC50 = 11.0 nM 7.96
CHEMBL2159804 IC50 = 12.0 nM 7.92
CHEMBL2159811 IC50 = 13.0 nM 7.89
CHEMBL2159795 IC50 = 19.0 nM 7.72
CHEMBL2159788 IC50 = 20.0 nM 7.70
CHEMBL2159797 IC50 = 21.0 nM 7.68
CHEMBL2159777 IC50 = 23.0 nM 7.64
CHEMBL2159782 IC50 = 30.0 nM 7.52
CHEMBL2159787 IC50 = 32.0 nM 7.50
CHEMBL2159778 IC50 = 33.0 nM 7.48
CHEMBL2159799 IC50 = 33.0 nM 7.48
CHEMBL2159809 IC50 = 36.0 nM 7.44
CHEMBL576786 IC50 = 37.0 nM 7.43
CHEMBL2159790 IC50 = 38.0 nM 7.42
CHEMBL2159794 IC50 = 41.0 nM 7.39
CHEMBL2159813 IC50 = 62.0 nM 7.21
CHEMBL2159789 IC50 = 63.0 nM 7.20
CHEMBL2159783 IC50 = 71.0 nM 7.15
CHEMBL2159808 IC50 = 102.0 nM 6.99
CHEMBL2159792 IC50 = 141.0 nM 6.85
CHEMBL2159791 IC50 = 162.0 nM 6.79
CHEMBL2159784 IC50 = 224.0 nM 6.65
CHEMBL2159793 IC50 = 234.0 nM 6.63
CHEMBL2159810 IC50 = 324.0 nM 6.49
CHEMBL2159785 IC50 = 372.0 nM 6.43
CHEMBL2159807 IC50 = 676.0 nM 6.17
CHEMBL2159786 IC50 = 955.0 nM 6.02
CHEMBL2159796 IC50 = 10471.0 nM 4.98