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Assay Detail

CHEMBL2162052

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine PNP using 7-methylguanosine as substrate by spectrophotometric based coupled assay
11
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Purine nucleoside phosphorylase (CHEMBL2935)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Trimeric purine nucleoside phosphorylase: exploring postulated one-third-of-the-sites binding in the transition state.
Wielgus-Kutrowska B, Breer K, Hashimoto M, Hikishima S, Yokomatsu T, Narczyk M, Dyzma A, Girstun A, Staroń K, Bzowska A.
Bioorg Med Chem (2012) 20 : 6758 -6769
PubMed 23040896 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kieq 9 - -
Ki 2 7.68 8.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL236709 2 8.16
CHEMBL2159650 2 7.20
CHEMBL367746 1 -
CHEMBL219568 GUANINE 3.0 1 -
CHEMBL1427 HYPOXANTHINE 1 -
CHEMBL1794646 1 -
CHEMBL236708 1 -
CHEMBL2159649 1 -
CHEMBL218291 FORODESINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL236709 Ki = 6.9 nM 8.16
CHEMBL2159650 Ki = 63.0 nM 7.20
CHEMBL367746 Kieq = 7400.0 nM -
CHEMBL219568 GUANINE Kieq = 4000.0 nM -
CHEMBL1427 HYPOXANTHINE Kieq = 2500.0 nM -
CHEMBL1794646 Kieq = 6000.0 nM -
CHEMBL2159650 Kieq = 63.0 nM -
CHEMBL236708 Kieq = 0.72 nM -
CHEMBL236709 Kieq = 0.085 nM -
CHEMBL2159649 Kieq = 6.8 nM -
CHEMBL218291 FORODESINE Kieq = 0.023 nM -