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Assay Detail

CHEMBL2162287

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human NPM-ALK phosphorylation using 4-MeUP substrate by cell based assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

NPM/ALK (Nucleophosmin/ALK tyrosine kinase receptor) (CHEMBL2111387)
Type CHIMERIC PROTEIN
Organism Homo sapiens

Publication

Strategies to mitigate the bioactivation of 2-anilino-7-aryl-pyrrolo[2,1-f][1,2,4]triazines: identification of orally bioavailable, efficacious ALK inhibitors.
Mesaros EF, Thieu TV, Wells GJ, Zificsak CA, Wagner JC, Breslin HJ, Tripathy R, Diebold JL, McHugh RJ, Wohler AT, Quail MR, Wan W, Lu L, Huang Z, Albom MS, Angeles TS, Wells-Knecht KJ, Aimone LD, Cheng M, Ator MA, Ott GR, Dorsey BD.
J Med Chem (2012) 55 : 115 -125
PubMed 22141319 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.01 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2158531 1 7.52
CHEMBL2158527 1 7.40
CHEMBL1822525 1 7.22
CHEMBL2158525 1 7.22
CHEMBL2158526 1 7.16
CHEMBL2158529 1 7.16
CHEMBL2158518 1 7.16
CHEMBL2158519 1 7.16
CHEMBL2158530 1 7.10
CHEMBL2158516 1 7.10
CHEMBL2158528 1 7.00
CHEMBL2158511 1 7.00
CHEMBL1934334 1 7.00
CHEMBL2158586 1 6.82
CHEMBL2158515 1 6.82
CHEMBL2158521 1 6.70
CHEMBL2158587 1 6.70
CHEMBL2158513 1 6.60
CHEMBL2158512 1 6.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2158531 IC50 = 30.0 nM 7.52
CHEMBL2158527 IC50 = 40.0 nM 7.40
CHEMBL1822525 IC50 = 60.0 nM 7.22
CHEMBL2158525 IC50 = 60.0 nM 7.22
CHEMBL2158526 IC50 = 70.0 nM 7.16
CHEMBL2158529 IC50 = 70.0 nM 7.16
CHEMBL2158518 IC50 = 70.0 nM 7.16
CHEMBL2158519 IC50 = 70.0 nM 7.16
CHEMBL2158530 IC50 = 80.0 nM 7.10
CHEMBL2158516 IC50 = 80.0 nM 7.10
CHEMBL2158528 IC50 = 100.0 nM 7.00
CHEMBL2158511 IC50 = 100.0 nM 7.00
CHEMBL1934334 IC50 = 100.0 nM 7.00
CHEMBL2158586 IC50 = 150.0 nM 6.82
CHEMBL2158515 IC50 = 150.0 nM 6.82
CHEMBL2158521 IC50 = 200.0 nM 6.70
CHEMBL2158587 IC50 = 200.0 nM 6.70
CHEMBL2158513 IC50 = 250.0 nM 6.60
CHEMBL2158512 IC50 = 500.0 nM 6.30