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Assay Detail

CHEMBL2162633

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal His8-tagged Bcl-XL expressed in Escherichia coli BL21(DE3) assessed as inhibition of Fluorescein-labeled BAK binding after 1 to 2 hrs by fluorescence polarization assay
30
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bcl-2-like protein 1 (CHEMBL4625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design of potent Bcl-2/Bcl-xL inhibitors with strong in vivo antitumor activity.
Zhou H, Aguilar A, Chen J, Bai L, Liu L, Meagher JL, Yang CY, McEachern D, Cong X, Stuckey JA, Wang S.
J Med Chem (2012) 55 : 6149 -6161
PubMed 22747598 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 6.15 8.92
IC50 15 7.91 8.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2159738 2 8.92
CHEMBL2159740 2 8.32
CHEMBL2159743 2 8.30
CHEMBL2159734 2 8.30
CHEMBL2159737 2 8.22
CHEMBL2159736 2 8.22
CHEMBL376408 ABT 737 1.0 2 8.22
CHEMBL2159739 2 8.18
CHEMBL2063897 2 8.12
CHEMBL2159741 2 8.11
CHEMBL2159735 2 8.05
CHEMBL2159744 2 5.47
CHEMBL2159733 2 4.06
CHEMBL2159742 2 -
CHEMBL2063880 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2159738 Ki = 1.2 nM 8.92
CHEMBL2159740 IC50 = 4.8 nM 8.32
CHEMBL2159743 IC50 = 5.0 nM 8.30
CHEMBL2159734 IC50 = 5.0 nM 8.30
CHEMBL2159737 IC50 = 6.0 nM 8.22
CHEMBL376408 ABT 737 IC50 = 6.0 nM 8.22
CHEMBL2159736 IC50 = 6.0 nM 8.22
CHEMBL2159739 IC50 = 6.6 nM 8.18
CHEMBL2063897 IC50 = 7.5 nM 8.12
CHEMBL2159741 IC50 = 7.7 nM 8.11
CHEMBL2159735 IC50 = 9.0 nM 8.05
CHEMBL2159738 IC50 = 11.0 nM 7.96
CHEMBL2159744 Ki = 3400.0 nM 5.47
CHEMBL2159744 IC50 = 11000.0 nM 4.96
CHEMBL2159733 Ki = 88000.0 nM 4.06
CHEMBL2159742 Ki < 1.0 nM -
CHEMBL2159741 Ki < 1.0 nM -
CHEMBL2159740 Ki < 1.0 nM -
CHEMBL2159739 Ki < 1.0 nM -
CHEMBL2159737 Ki < 1.0 nM -
CHEMBL2159736 Ki < 1.0 nM -
CHEMBL376408 ABT 737 Ki < 1.0 nM -
CHEMBL2159735 Ki < 1.0 nM -
CHEMBL2159734 Ki < 1.0 nM -
CHEMBL2063897 Ki < 1.0 nM -
CHEMBL2159742 IC50 < 1.0 nM -
CHEMBL2159733 IC50 = 291000.0 nM -
CHEMBL2063880 IC50 = 453000.0 nM -
CHEMBL2063880 Ki = 138000.0 nM -
CHEMBL2159743 Ki < 1.0 nM -