Assay Detail
Binding
CHEMBL2162713
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to wild type Smo expressed in U2OS cells by scintillation counting
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | U2OS |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Identification of a novel Smoothened antagonist that potently suppresses Hedgehog signaling.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 1 | 7.91 | 7.91 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL254129 | CYCLOPAMINE | — | 1 | 7.91 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL254129 | CYCLOPAMINE | Kd | = | 12.4 | nM | 7.91 |