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Assay Detail

CHEMBL2162785

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of GAL4-fused PPARalpha ligand binding domain transfected in human HepG2 cells after 20 hrs by luciferase reporter gene assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor alpha (CHEMBL239)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Diarylheptanoid glycosides from Tacca plantaginea and their effects on NF-κB activation and PPAR transcriptional activity.
Quang TH, Ngan NT, Minh CV, Kiem PV, Yen PH, Tai BH, Nhiem NX, Thao NP, Anh Hle T, Luyen BT, Yang SY, Choi CW, Kim YH.
Bioorg Med Chem Lett (2012) 22 : 6681 -6687
PubMed 23031596 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 4.92 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL557555 CIPROFIBRATE 4.0 1 6.05
CHEMBL458899 1 4.37
CHEMBL2159605 PLANTAGINEOSIDE A 1 4.33
CHEMBL465800 1 -
CHEMBL516827 1 -
CHEMBL2159607 PLANTAGINEOSIDE C 1 -
CHEMBL2159606 PLANTAGINEOSIDE B 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL557555 CIPROFIBRATE EC50 = 900.0 nM 6.05
CHEMBL458899 EC50 = 42900.0 nM 4.37
CHEMBL2159605 PLANTAGINEOSIDE A EC50 = 47100.0 nM 4.33
CHEMBL465800 EC50 > 50000.0 nM -
CHEMBL516827 EC50 > 50000.0 nM -
CHEMBL2159607 PLANTAGINEOSIDE C EC50 > 50000.0 nM -
CHEMBL2159606 PLANTAGINEOSIDE B EC50 > 50000.0 nM -