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Assay Detail

CHEMBL2167891

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Functional
Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type COS-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuropeptide FF receptor 1 (CHEMBL5951)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective mode of action of guanidine-containing non-peptides at human NPFF receptors.
Findeisen M, Würker C, Rathmann D, Meier R, Meiler J, Olsson R, Beck-Sickinger AG.
J Med Chem (2012) 55 : 6124 -6136
PubMed 22708927 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.61 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2165920 1 8.77
CHEMBL1672380 1 7.15
CHEMBL573297 1 6.28
CHEMBL583068 1 5.62
CHEMBL2165921 1 5.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2165920 EC50 = 1.7 nM 8.77
CHEMBL1672380 EC50 = 71.0 nM 7.15
CHEMBL573297 EC50 = 527.0 nM 6.28
CHEMBL583068 EC50 = 2370.0 nM 5.62
CHEMBL2165921 EC50 = 5624.0 nM 5.25