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Assay Detail

CHEMBL2174353

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-His-ghrelin from human ghrelin receptor expressed in COS7 cells incubated for 75 mins by scintillation counting based assay
10
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Growth hormone secretagogue receptor type 1 (CHEMBL4616)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

An aromatic region to induce a switch between agonism and inverse agonism at the ghrelin receptor.
Els S, Schild E, Petersen PS, Kilian TM, Mokrosinski J, Frimurer TM, Chollet C, Schwartz TW, Holst B, Beck-Sickinger AG.
J Med Chem (2012) 55 : 7437 -7449
PubMed 22920150 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 7.62 9.15
IC50 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL500468 GHRELIN 3.0 1 9.15
CHEMBL2170781 1 8.31
CHEMBL2170701 1 7.66
CHEMBL2170693 1 7.50
CHEMBL2170694 1 7.46
CHEMBL2170779 1 6.63
CHEMBL2170780 1 6.61
CHEMBL2170692 1 -
CHEMBL2170691 1 -
CHEMBL2170690 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL500468 GHRELIN Ki = 0.7 nM 9.15
CHEMBL2170781 Ki = 4.9 nM 8.31
CHEMBL2170701 Ki = 22.1 nM 7.66
CHEMBL2170693 Ki = 31.3 nM 7.50
CHEMBL2170694 Ki = 34.9 nM 7.46
CHEMBL2170779 Ki = 234.2 nM 6.63
CHEMBL2170780 Ki = 247.2 nM 6.61
CHEMBL2170692 IC50 >= 1000.0 nM -
CHEMBL2170691 IC50 >= 1000.0 nM -
CHEMBL2170690 IC50 >= 1000.0 nM -