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Assay Detail

CHEMBL2174887

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]CGS21680 from human A2a adenosine receptor expressed in HEK293 cells after 60 min by Perkin Elmer Liquid Scintillation Analyzer
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural sweet spot for A1 adenosine receptor activation by truncated (N)-methanocarba nucleosides: receptor docking and potent anticonvulsant activity.
Tosh DK, Paoletta S, Deflorian F, Phan K, Moss SM, Gao ZG, Jiang X, Jacobson KA.
J Med Chem (2012) 55 : 8075 -8090
PubMed 22921089 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 5.68 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL464859 NECA 1 7.70
CHEMBL1512545 1 6.43
CHEMBL68738 N6-CYCLOPENTYLADENOSINE 1 6.10
CHEMBL2170787 1 5.80
CHEMBL2170796 1 5.65
CHEMBL284969 1 5.64
CHEMBL2170792 1 5.59
CHEMBL2012672 1 5.50
CHEMBL2170788 1 5.44
CHEMBL2170785 1 5.44
CHEMBL2012686 1 5.40
CHEMBL2170798 1 5.39
CHEMBL2012684 1 5.36
CHEMBL2170791 1 5.32
CHEMBL2170786 1 5.31
CHEMBL2170789 1 5.26
CHEMBL2170790 1 5.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL464859 NECA Ki = 20.0 nM 7.70
CHEMBL1512545 Ki = 370.0 nM 6.43
CHEMBL68738 N6-CYCLOPENTYLADENOSINE Ki = 794.0 nM 6.10
CHEMBL2170787 Ki = 1570.0 nM 5.80
CHEMBL2170796 Ki = 2230.0 nM 5.65
CHEMBL284969 Ki = 2270.0 nM 5.64
CHEMBL2170792 Ki = 2550.0 nM 5.59
CHEMBL2012672 Ki = 3140.0 nM 5.50
CHEMBL2170788 Ki = 3650.0 nM 5.44
CHEMBL2170785 Ki = 3610.0 nM 5.44
CHEMBL2012686 Ki = 3950.0 nM 5.40
CHEMBL2170798 Ki = 4110.0 nM 5.39
CHEMBL2012684 Ki = 4410.0 nM 5.36
CHEMBL2170791 Ki = 4840.0 nM 5.32
CHEMBL2170786 Ki = 4910.0 nM 5.31
CHEMBL2170789 Ki = 5470.0 nM 5.26
CHEMBL2170790 Ki = 6450.0 nM 5.19