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Assay Detail

CHEMBL2176381

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DAGLalpha expressed in HEK293T cell membrane using [14C]SAG substrate in detergent free solution by FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Diacylglycerol lipase-alpha (CHEMBL5545)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Assay and inhibition of diacylglycerol lipase activity.
Johnston M, Bhatt SR, Sikka S, Mercier RW, West JM, Makriyannis A, Gatley SJ, Duclos RI.
Bioorg Med Chem Lett (2012) 22 : 4585 -4592
PubMed 22738638 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.00 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL175247 ORLISTAT 4.0 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL175247 ORLISTAT IC50 = 10.0 nM 8.00