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Assay Detail

CHEMBL2187905

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human BRD2 assessed as inhibition of Alexa Fluor 488 binding after 60 mins by fluorescence anisotropic analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 2 (CHEMBL1293289)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a novel series of BET family bromodomain inhibitors: binding mode and profile of I-BET151 (GSK1210151A).
Seal J, Lamotte Y, Donche F, Bouillot A, Mirguet O, Gellibert F, Nicodeme E, Krysa G, Kirilovsky J, Beinke S, McCleary S, Rioja I, Bamborough P, Chung CW, Gordon L, Lewis T, Walker AL, Cutler L, Lugo D, Wilson DM, Witherington J, Lee K, Prinjha RK.
Bioorg Med Chem Lett (2012) 22 : 2968 -2972
PubMed 22437115 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.90 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2017288 1 6.40
CHEMBL2017291 1 6.30
CHEMBL2017285 1 6.30
CHEMBL2017283 1 6.30
CHEMBL2017273 1 6.30
CHEMBL2017269 1 6.10
CHEMBL2017281 1 6.00
CHEMBL2181820 1 5.80
CHEMBL2017286 1 5.80
CHEMBL2181818 1 5.80
CHEMBL2017289 1 5.70
CHEMBL2017277 1 5.70
CHEMBL2017282 1 5.60
CHEMBL2181819 1 5.50
CHEMBL2017264 1 4.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2017288 IC50 = 398.11 nM 6.40
CHEMBL2017291 IC50 = 501.19 nM 6.30
CHEMBL2017285 IC50 = 501.19 nM 6.30
CHEMBL2017283 IC50 = 501.19 nM 6.30
CHEMBL2017273 IC50 = 501.19 nM 6.30
CHEMBL2017269 IC50 = 794.33 nM 6.10
CHEMBL2017281 IC50 = 1000.0 nM 6.00
CHEMBL2181820 IC50 = 1584.89 nM 5.80
CHEMBL2017286 IC50 = 1584.89 nM 5.80
CHEMBL2181818 IC50 = 1584.89 nM 5.80
CHEMBL2017289 IC50 = 1995.26 nM 5.70
CHEMBL2017277 IC50 = 1995.26 nM 5.70
CHEMBL2017282 IC50 = 2511.89 nM 5.60
CHEMBL2181819 IC50 = 3162.28 nM 5.50
CHEMBL2017264 IC50 = 12589.25 nM 4.90