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Assay Detail

CHEMBL2189067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human kidney glutaminase (124 to 669) using L-[3H]-glutamine as substrate after 45 mins by GLS assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glutaminase kidney isoform, mitochondrial (CHEMBL2146302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and pharmacological evaluation of bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide 3 (BPTES) analogs as glutaminase inhibitors.
Shukla K, Ferraris DV, Thomas AG, Stathis M, Duvall B, Delahanty G, Alt J, Rais R, Rojas C, Gao P, Xiang Y, Dang CV, Slusher BS, Tsukamoto T.
J Med Chem (2012) 55 : 10551 -10563
PubMed 23151085 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 5.06 5.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2177749 1 5.85
CHEMBL2178396 1 5.72
CHEMBL2177740 1 5.58
CHEMBL2178393 1 5.57
CHEMBL2177757 1 5.48
CHEMBL2177748 1 5.42
CHEMBL2177739 1 5.35
CHEMBL2177744 1 5.34
CHEMBL2177753 1 5.28
CHEMBL2178398 1 5.14
CHEMBL2177746 1 5.09
CHEMBL2177747 1 5.00
CHEMBL2177752 1 4.96
CHEMBL2177741 1 4.92
CHEMBL2178395 1 4.82
CHEMBL2177738 1 4.66
CHEMBL2177751 1 4.58
CHEMBL2177755 1 4.32
CHEMBL2178394 1 4.21
CHEMBL2177758 1 4.00
CHEMBL2177742 1 -
CHEMBL2177743 1 -
CHEMBL2178397 1 -
CHEMBL2177745 1 -
CHEMBL2177750 1 -
CHEMBL2177754 1 -
CHEMBL2177756 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2177749 IC50 = 1400.0 nM 5.85
CHEMBL2178396 IC50 = 1900.0 nM 5.72
CHEMBL2177740 IC50 = 2600.0 nM 5.58
CHEMBL2178393 IC50 = 2700.0 nM 5.57
CHEMBL2177757 IC50 = 3300.0 nM 5.48
CHEMBL2177748 IC50 = 3800.0 nM 5.42
CHEMBL2177739 IC50 = 4500.0 nM 5.35
CHEMBL2177744 IC50 = 4600.0 nM 5.34
CHEMBL2177753 IC50 = 5200.0 nM 5.28
CHEMBL2178398 IC50 = 7200.0 nM 5.14
CHEMBL2177746 IC50 = 8100.0 nM 5.09
CHEMBL2177747 IC50 = 10000.0 nM 5.00
CHEMBL2177752 IC50 = 11000.0 nM 4.96
CHEMBL2177741 IC50 = 12000.0 nM 4.92
CHEMBL2178395 IC50 = 15000.0 nM 4.82
CHEMBL2177738 IC50 = 22000.0 nM 4.66
CHEMBL2177751 IC50 = 26000.0 nM 4.58
CHEMBL2177755 IC50 = 48000.0 nM 4.32
CHEMBL2178394 IC50 = 61000.0 nM 4.21
CHEMBL2177758 IC50 = 100000.0 nM 4.00
CHEMBL2177742 IC50 > 100000.0 nM -
CHEMBL2177743 IC50 > 100000.0 nM -
CHEMBL2178397 IC50 > 100000.0 nM -
CHEMBL2177745 IC50 > 100000.0 nM -
CHEMBL2177750 IC50 > 100000.0 nM -
CHEMBL2177754 IC50 > 100000.0 nM -
CHEMBL2177756 IC50 > 100000.0 nM -