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Assay Detail

CHEMBL2213717

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HCV genotype 1b BK NS3/4A protease D168V mutant expressed in Escherichia coli incubated for 30 mins by time-resolved fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus (isolate BK)
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Genome polyprotein (CHEMBL4296298)
Type SINGLE PROTEIN
Organism Hepacivirus C

Publication

Development of macrocyclic inhibitors of HCV NS3/4A protease with cyclic constrained P2-P4 linkers.
Rudd MT, McIntyre CJ, Romano JJ, Butcher JW, Holloway MK, Bush K, Nguyen KT, Gilbert KF, Lyle TA, Liverton NJ, Wan BL, Summa V, Harper S, Rowley M, Vacca JP, Carroll SS, Burlein C, DiMuzio JM, Gates A, Graham DJ, Huang Q, Ludmerer SW, McClain S, McHale C, Stahlhut M, Fandozzi C, Taylor A, Trainor N, Olsen DB, McCauley JA.
Bioorg Med Chem Lett (2012) 22 : 7207 -7213
PubMed 23084906 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 8.77 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2203892 1 8.92
CHEMBL2203882 1 8.74
CHEMBL2203873 1 8.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2203892 Ki = 1.2 nM 8.92
CHEMBL2203882 Ki = 1.8 nM 8.74
CHEMBL2203873 Ki = 2.2 nM 8.66