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Assay Detail

CHEMBL2215060

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of radioligand [3H]osanetant at human NK3 H316W mutant expressed in HEK293 cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuromedin-K receptor (CHEMBL4429)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a crucial amino acid in the helix position 6.51 of human tachykinin neurokinin 1 and 3 receptors contributing to the insurmountable mode of antagonism by dual NK₁/NK₃ antagonists.
Bissantz C, Bohnert C, Hoffmann T, Marcuz A, Schnider P, Malherbe P.
J Med Chem (2012) 55 : 5061 -5076
PubMed 22574973 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 6.98 8.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL346178 OSANETANT 2.0 1 8.32
CHEMBL10188 TALNETANT 2.0 1 6.91
CHEMBL2203703 1 5.70
CHEMBL2203704 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL346178 OSANETANT Ki = 4.8 nM 8.32
CHEMBL10188 TALNETANT Ki = 122.0 nM 6.91
CHEMBL2203703 Ki = 2010.0 nM 5.70
CHEMBL2203704 Ki > 10000.0 nM -