Assay Detail

CHEMBL2215514

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional

Antiproliferative activity against human HN5 cells overexpressing EGFR after 48 hrs by BrdU labeling assay

ChEMBL ↗

Free Research Context This assay will appear in recent viewed items on this browser. Open the demo workspace to preview project-only review queues.

Research home Demo workspace

Target

HN5 (CHEMBL614828) ↗

Open DB target context

Use one target anchor to keep assay intent, target linkage, and tested compound review aligned.

Loading context

HN5 (CHEMBL614828) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target HN5

CHEMBL614828

Anchored from Assay target.

Protein identity HN5

Disease frame Disease evidence pending

Open the live target snapshot or evidence card before reviewing assay metadata so target fit and disease framing stay reusable.

Review focus Open DB context pending

Open the live target snapshot before relying on this target frame across surface handoff.

Open target snapshot Open evidence card Search with this context

Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Functional
Organism	Homo sapiens
Cell Type	HN5
Confidence	1 — Organism
Curated By	Autocuration

Target

HN5 (CHEMBL614828) ↗

Type	CELL-LINE
Organism	Homo sapiens

Publication

Discovery of XL888: a novel tropane-derived small molecule inhibitor of HSP90.

Bussenius J, Blazey CM, Aay N, Anand NK, Arcalas A, Baik T, Bowles OJ, Buhr CA, Costanzo S, Curtis JK, DeFina SC, Dubenko L, Heuer TS, Huang P, Jaeger C, Joshi A, Kennedy AR, Kim AI, Lara K, Lee J, Li J, Lougheed JC, Ma S, Malek S, Manalo JC, Martini JF, McGrath G, Nicoll M, Nuss JM, Pack M, Peto CJ, Tsang TH, Wang L, Womble SW, Yakes M, Zhang W, Rice KD.
Bioorg Med Chem Lett (2012) 22 : 5396 -5404
PubMed 22877636 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	1	8.26	8.26

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL2204502	—	—	1	8.26

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL2204502	—	IC50	=	5.5	nM	8.26