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Assay Detail

CHEMBL2215698

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of bovine pancreatic RNase A type 12-A assessed as degradation of yeast tRNA preincubated for 10 mins before addition of the enzyme by double reciprocal plot analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Triazole pyrimidine nucleosides as inhibitors of Ribonuclease A. Synthesis, biochemical, and structural evaluation.
Parmenopoulou V, Chatzileontiadou DS, Manta S, Bougiatioti S, Maragozidis P, Gkaragkouni DN, Kaffesaki E, Kantsadi AL, Skamnaki VT, Zographos SE, Zounpoulakis P, Balatsos NA, Komiotis D, Leonidas DD.
Bioorg Med Chem (2012) 20 : 7184 -7193
PubMed 23122937 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 4.53 5.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2206660 1 5.80
CHEMBL2206117 1 4.59
CHEMBL100259 URIDINE 3.0 1 4.54
CHEMBL2206657 1 4.51
CHEMBL54918 5-FLUOROURIDINE 2.0 1 4.47
CHEMBL106175 1 4.36
CHEMBL2206659 1 4.35
CHEMBL2206658 1 4.16
CHEMBL2206656 1 4.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2206660 Ki = 1600.0 nM 5.80
CHEMBL2206117 Ki = 25800.0 nM 4.59
CHEMBL100259 URIDINE Ki = 28500.0 nM 4.54
CHEMBL2206657 Ki = 30800.0 nM 4.51
CHEMBL54918 5-FLUOROURIDINE Ki = 33600.0 nM 4.47
CHEMBL106175 Ki = 44100.0 nM 4.36
CHEMBL2206659 Ki = 44500.0 nM 4.35
CHEMBL2206658 Ki = 69100.0 nM 4.16
CHEMBL2206656 Ki = 94800.0 nM 4.02