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Assay Detail

CHEMBL2319595

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant MAGL using [3H]-2-OG as substrate incubated for 30 mins at room temperature followed by incubation at 37 degC for 10 mins by liquid scintillation assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Monoglyceride lipase (CHEMBL4191)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

An unprecedented reversible mode of action of β-lactams for the inhibition of human fatty acid amide hydrolase (hFAAH).
Feledziak M, Michaux C, Lambert DM, Marchand-Brynaert J.
Eur J Med Chem (2013) 60 : 101 -111
PubMed 23287055 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 4.67 5.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2151432 1 5.39
CHEMBL2313948 1 4.99
CHEMBL2313951 1 4.72
CHEMBL2313941 1 4.25
CHEMBL2313949 1 4.01
CHEMBL2313947 1 -
CHEMBL2313945 1 -
CHEMBL2313944 1 -
CHEMBL2313943 1 -
CHEMBL2313942 1 -
CHEMBL2313940 1 -
CHEMBL2313952 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2151432 IC50 = 4060.0 nM 5.39
CHEMBL2313948 IC50 = 10300.0 nM 4.99
CHEMBL2313951 IC50 = 18960.0 nM 4.72
CHEMBL2313941 IC50 = 56850.0 nM 4.25
CHEMBL2313949 IC50 = 98410.0 nM 4.01
CHEMBL2313947 IC50 - -
CHEMBL2313945 IC50 - -
CHEMBL2313944 IC50 - -
CHEMBL2313943 IC50 - -
CHEMBL2313942 IC50 = 121700.0 nM -
CHEMBL2313940 IC50 - -
CHEMBL2313952 IC50 - -