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Assay Detail

CHEMBL2328496

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Beta-lactamase SHV-1 K234R protein mutant in Escherichia coli DH10B by Henri-Michaelis-Menten steady state equation analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design and exploration of novel boronic acid inhibitors reveals important interactions with a clavulanic acid-resistant sulfhydryl-variable (SHV) β-lactamase.
Winkler ML, Rodkey EA, Taracila MA, Drawz SM, Bethel CR, Papp-Wallace KM, Smith KM, Xu Y, Dwulit-Smith JR, Romagnoli C, Caselli E, Prati F, van den Akker F, Bonomo RA.
J Med Chem (2013) 56 : 1084 -1097
PubMed 23252553 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.56 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2326847 1 8.62
CHEMBL1277225 1 7.92
CHEMBL2326848 1 7.87
CHEMBL2326846 1 7.32
CHEMBL404 TAZOBACTAM 4.0 1 6.05
CHEMBL403 SULBACTAM 4.0 1 5.34
CHEMBL777 CLAVULANIC ACID 4.0 1 5.11
CHEMBL320049 1 4.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2326847 Ki = 2.4 nM 8.62
CHEMBL1277225 Ki = 12.0 nM 7.92
CHEMBL2326848 Ki = 13.5 nM 7.87
CHEMBL2326846 Ki = 48.0 nM 7.32
CHEMBL404 TAZOBACTAM Ki = 900.0 nM 6.05
CHEMBL403 SULBACTAM Ki = 4600.0 nM 5.34
CHEMBL777 CLAVULANIC ACID Ki = 7700.0 nM 5.11
CHEMBL320049 Ki = 61000.0 nM 4.21