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Assay Detail

CHEMBL2329833

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse MC3 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 3 (CHEMBL4774)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Structure-activity relationships of peptides incorporating a bioactive reverse-turn heterocycle at the melanocortin receptors: identification of a 5800-fold mouse melanocortin-3 receptor (mMC3R) selective antagonist/partial agonist versus the mouse melanocortin-4 receptor (mMC4R).
Singh A, Dirain M, Witek R, Rocca JR, Edison AS, Haskell-Luevano C.
J Med Chem (2013) 56 : 2747 -2763
PubMed 23432160 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 18 6.27 9.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 9.64
CHEMBL1688107 1 8.77
CHEMBL2323795 1 7.31
CHEMBL2323786 1 6.75
CHEMBL2323793 1 6.72
CHEMBL2323785 1 6.70
CHEMBL2323787 1 6.64
CHEMBL1688111 1 6.49
CHEMBL2323799 1 5.74
CHEMBL2323790 1 5.72
CHEMBL2323529 1 5.65
CHEMBL2323798 1 5.59
CHEMBL2323788 1 5.40
CHEMBL2323789 1 5.35
CHEMBL2323800 1 5.15
CHEMBL2323792 1 5.11
CHEMBL2323797 1 5.09
CHEMBL2323791 1 4.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE EC50 = 0.23 nM 9.64
CHEMBL1688107 EC50 = 1.7 nM 8.77
CHEMBL2323795 EC50 = 49.0 nM 7.31
CHEMBL2323786 EC50 = 180.0 nM 6.75
CHEMBL2323793 EC50 = 190.0 nM 6.72
CHEMBL2323785 EC50 = 200.0 nM 6.70
CHEMBL2323787 EC50 = 230.0 nM 6.64
CHEMBL1688111 EC50 = 324.0 nM 6.49
CHEMBL2323799 EC50 = 1830.0 nM 5.74
CHEMBL2323790 EC50 = 1900.0 nM 5.72
CHEMBL2323529 EC50 = 2230.0 nM 5.65
CHEMBL2323798 EC50 = 2540.0 nM 5.59
CHEMBL2323788 EC50 = 4020.0 nM 5.40
CHEMBL2323789 EC50 = 4500.0 nM 5.35
CHEMBL2323800 EC50 = 7120.0 nM 5.15
CHEMBL2323792 EC50 = 7720.0 nM 5.11
CHEMBL2323797 EC50 = 8070.0 nM 5.09
CHEMBL2323791 EC50 = 10350.0 nM 4.99