Assay Detail
Binding
CHEMBL2330739
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to Enterobacteria phage T4 lysozyme L99A/M102E double mutant expressed in Escherichia coli BL21(DE3) by isothermal titration calorimetry
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Tequatrovirus T4 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
The impact of introducing a histidine into an apolar cavity site on docking and ligand recognition.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 6 | 4.36 | 4.57 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL277500 | BENZENE | — | 1 | 4.57 |
| CHEMBL371561 | ETHYLBENZENE | — | 1 | 4.36 |
| CHEMBL9113 | TOLUENE | 2.0 | 1 | 4.15 |
| CHEMBL31561 | P-XYLENE | — | 1 | - |
| CHEMBL14060 | PHENOL | 4.0 | 1 | - |
| CHEMBL14130 | P-NITROPHENOL | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL277500 | BENZENE | Kd | = | 27000.0 | nM | 4.57 |
| CHEMBL371561 | ETHYLBENZENE | Kd | = | 44000.0 | nM | 4.36 |
| CHEMBL9113 | TOLUENE | Kd | = | 71000.0 | nM | 4.15 |
| CHEMBL31561 | P-XYLENE | Kd | = | 151000.0 | nM | - |
| CHEMBL14060 | PHENOL | Kd | = | 109000.0 | nM | - |
| CHEMBL14130 | P-NITROPHENOL | Kd | - | — | - |