Assay Detail
Binding
CHEMBL2330741
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to Enterobacteria phage T4 lysozyme L99A mutant expressed in Escherichia coli BL21(DE3) by isothermal titration calorimetry
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Tequatrovirus T4 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
The impact of introducing a histidine into an apolar cavity site on docking and ligand recognition.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 6 | 4.17 | 4.17 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL371561 | ETHYLBENZENE | — | 1 | 4.17 |
| CHEMBL31561 | P-XYLENE | — | 1 | - |
| CHEMBL9113 | TOLUENE | 2.0 | 1 | - |
| CHEMBL277500 | BENZENE | — | 1 | - |
| CHEMBL14060 | PHENOL | 4.0 | 1 | - |
| CHEMBL14130 | P-NITROPHENOL | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL371561 | ETHYLBENZENE | Kd | = | 68000.0 | nM | 4.17 |
| CHEMBL31561 | P-XYLENE | Kd | = | 422000.0 | nM | - |
| CHEMBL9113 | TOLUENE | Kd | = | 102000.0 | nM | - |
| CHEMBL277500 | BENZENE | Kd | = | 175000.0 | nM | - |
| CHEMBL14060 | PHENOL | Kd | - | — | - | |
| CHEMBL14130 | P-NITROPHENOL | Kd | - | — | - |