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Assay Detail

CHEMBL2330770

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-Epibatidine from rat alpha3beta2 nACHR expressed in HEK293 cell membranes by liquid scintillation counting analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha3/beta2 (CHEMBL1907587)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Chemistry and pharmacological studies of 3-alkoxy-2,5-disubstituted-pyridinyl compounds as novel selective α4β2 nicotinic acetylcholine receptor ligands that reduce alcohol intake in rats.
Liu Y, Richardson J, Tran T, Al-Muhtasib N, Xie T, Yenugonda VM, Sexton HG, Rezvani AH, Levin ED, Sahibzada N, Kellar KJ, Brown ML, Xiao Y, Paige M.
J Med Chem (2013) 56 : 3000 -3011
PubMed 23540678 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 6.71 9.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2024096 1 9.42
CHEMBL1076903 VARENICLINE 4.0 1 8.60
CHEMBL3 NICOTINE 4.0 1 7.33
CHEMBL2323570 1 6.66
CHEMBL2323571 1 6.64
CHEMBL2323572 1 6.52
CHEMBL2323569 1 6.47
CHEMBL2323566 1 6.41
CHEMBL2323568 1 5.60
CHEMBL2323565 1 5.44
CHEMBL2323567 1 4.72
CHEMBL2323573 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2024096 Ki = 0.38 nM 9.42
CHEMBL1076903 VARENICLINE Ki = 2.5 nM 8.60
CHEMBL3 NICOTINE Ki = 47.0 nM 7.33
CHEMBL2323570 Ki = 220.0 nM 6.66
CHEMBL2323571 Ki = 230.0 nM 6.64
CHEMBL2323572 Ki = 300.0 nM 6.52
CHEMBL2323569 Ki = 340.0 nM 6.47
CHEMBL2323566 Ki = 390.0 nM 6.41
CHEMBL2323568 Ki = 2500.0 nM 5.60
CHEMBL2323565 Ki = 3600.0 nM 5.44
CHEMBL2323567 Ki = 19000.0 nM 4.72
CHEMBL2323573 Ki - -