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Assay Detail

CHEMBL2340159

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Binding
Agonist activity at beta2-adrenoceptor in guinea pig trachea assessed as relaxation of histamine-induced precontracted tracheal rings
27
Total Activities
9
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Trachea
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL5414)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Hybrids consisting of the pharmacophores of salmeterol and roflumilast or phthalazinone: dual β₂-adrenoceptor agonists-PDE4 inhibitors for the treatment of COPD.
Liu A, Huang L, Wang Z, Luo Z, Mao F, Shan W, Xie J, Lai K, Li X.
Bioorg Med Chem Lett (2013) 23 : 1548 -1552
PubMed 23375225 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Activity 9 - -
Emax 9 - -
EC50 9 8.71 9.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2335415 3 9.20
CHEMBL2335412 3 9.19
CHEMBL2335413 3 9.17
CHEMBL2335414 3 9.08
CHEMBL2335410 3 8.71
CHEMBL2335409 3 8.55
CHEMBL2335411 3 8.50
CHEMBL2335408 3 8.47
CHEMBL434 ISOPROTERENOL 4.0 3 7.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2335415 EC50 = 0.631 nM 9.20
CHEMBL2335412 EC50 = 0.6457 nM 9.19
CHEMBL2335413 EC50 = 0.6761 nM 9.17
CHEMBL2335414 EC50 = 0.8318 nM 9.08
CHEMBL2335410 EC50 = 1.95 nM 8.71
CHEMBL2335409 EC50 = 2.818 nM 8.55
CHEMBL2335411 EC50 = 3.162 nM 8.50
CHEMBL2335408 EC50 = 3.388 nM 8.47
CHEMBL434 ISOPROTERENOL EC50 = 28.84 nM 7.54
CHEMBL2335414 Activity - -
CHEMBL2335413 Activity - -
CHEMBL2335412 Activity - -
CHEMBL2335411 Activity - -
CHEMBL2335410 Activity - -
CHEMBL2335409 Activity - -
CHEMBL2335408 Activity - -
CHEMBL434 ISOPROTERENOL Activity - -
CHEMBL2335415 Emax = 102.0 % -
CHEMBL2335414 Emax = 100.0 % -
CHEMBL2335413 Emax = 100.0 % -
CHEMBL2335412 Emax = 102.0 % -
CHEMBL2335415 Activity - -
CHEMBL2335410 Emax = 103.0 % -
CHEMBL2335409 Emax = 103.0 % -
CHEMBL2335408 Emax = 100.0 % -
CHEMBL434 ISOPROTERENOL Emax = 100.0 % -
CHEMBL2335411 Emax = 105.0 % -