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Assay Detail

CHEMBL2343386

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Ex vivo activation of TGR5 in human PBMC assessed as elevation of cAMP level by cAMP assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 1 — Organism
Curated By Autocuration

Target

Homo sapiens (CHEMBL372)
Type ORGANISM
Organism Homo sapiens

Publication

Discovery of 5-phenoxy-1,3-dimethyl-1H-pyrazole-4-carboxamides as potent agonists of TGR5 via sequential combinatorial libraries.
Londregan AT, Piotrowski DW, Futatsugi K, Warmus JS, Boehm M, Carpino PA, Chin JE, Janssen AM, Roush NS, Buxton J, Hinchey T.
Bioorg Med Chem Lett (2013) 23 : 1407 -1411
PubMed 23337601 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 6.53 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2331648 1 6.96
CHEMBL2336222 1 6.42
CHEMBL576138 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2331648 EC50 = 110.0 nM 6.96
CHEMBL2336222 EC50 = 379.0 nM 6.42
CHEMBL576138 EC50 = 600.0 nM 6.22