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Assay Detail

CHEMBL2344827

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FLT3-ITD mutant in human MV4-11 cells assessed as decrease in cell viability after 72 hrs by MTT assay
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MV4-11
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship studies of pyrazolo[3,4-d]pyrimidine derivatives leading to the discovery of a novel multikinase inhibitor that potently inhibits FLT3 and VEGFR2 and evaluation of its activity against acute myeloid leukemia in vitro and in vivo.
Yang LL, Li GB, Ma S, Zou C, Zhou S, Sun QZ, Cheng C, Chen X, Wang LJ, Feng S, Li LL, Yang SY.
J Med Chem (2013) 56 : 1641 -1655
PubMed 23362959 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 6.79 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2331570 1 8.40
CHEMBL2332840 1 8.40
CHEMBL2332849 1 8.30
CHEMBL1336 SORAFENIB 4.0 1 8.15
CHEMBL2332867 1 7.96
CHEMBL2332870 1 7.77
CHEMBL2332871 1 7.75
CHEMBL2332846 1 7.70
CHEMBL2332848 1 7.43
CHEMBL2332869 1 7.27
CHEMBL2332868 1 7.23
CHEMBL2332851 1 7.10
CHEMBL2332847 1 7.08
CHEMBL2332866 1 7.05
CHEMBL2332865 1 7.02
CHEMBL2332844 1 6.58
CHEMBL2332860 1 6.50
CHEMBL2332864 1 6.44
CHEMBL2332861 1 6.35
CHEMBL2332862 1 5.97
CHEMBL2332842 1 5.91
CHEMBL2332863 1 5.86
CHEMBL2332850 1 5.80
CHEMBL2332841 1 5.78
CHEMBL2332843 1 5.77
CHEMBL2332845 1 5.69
CHEMBL2332852 1 5.34
CHEMBL2332856 1 5.15
CHEMBL2332853 1 5.11
CHEMBL2332854 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2331570 IC50 = 4.0 nM 8.40
CHEMBL2332840 IC50 = 4.0 nM 8.40
CHEMBL2332849 IC50 = 5.0 nM 8.30
CHEMBL1336 SORAFENIB IC50 = 7.0 nM 8.15
CHEMBL2332867 IC50 = 11.0 nM 7.96
CHEMBL2332870 IC50 = 17.0 nM 7.77
CHEMBL2332871 IC50 = 18.0 nM 7.75
CHEMBL2332846 IC50 = 20.0 nM 7.70
CHEMBL2332848 IC50 = 37.0 nM 7.43
CHEMBL2332869 IC50 = 54.0 nM 7.27
CHEMBL2332868 IC50 = 59.0 nM 7.23
CHEMBL2332851 IC50 = 79.0 nM 7.10
CHEMBL2332847 IC50 = 83.0 nM 7.08
CHEMBL2332866 IC50 = 90.0 nM 7.05
CHEMBL2332865 IC50 = 96.0 nM 7.02
CHEMBL2332844 IC50 = 260.0 nM 6.58
CHEMBL2332860 IC50 = 320.0 nM 6.50
CHEMBL2332864 IC50 = 363.0 nM 6.44
CHEMBL2332861 IC50 = 449.0 nM 6.35
CHEMBL2332862 IC50 = 1074.0 nM 5.97
CHEMBL2332842 IC50 = 1240.0 nM 5.91
CHEMBL2332863 IC50 = 1371.0 nM 5.86
CHEMBL2332850 IC50 = 1590.0 nM 5.80
CHEMBL2332841 IC50 = 1650.0 nM 5.78
CHEMBL2332843 IC50 = 1690.0 nM 5.77
CHEMBL2332845 IC50 = 2050.0 nM 5.69
CHEMBL2332852 IC50 = 4530.0 nM 5.34
CHEMBL2332856 IC50 = 7070.0 nM 5.15
CHEMBL2332853 IC50 = 7820.0 nM 5.11
CHEMBL2332854 IC50 > 10000.0 nM -
CHEMBL2332855 IC50 > 10000.0 nM -
CHEMBL2332857 IC50 > 10000.0 nM -
CHEMBL2332858 IC50 > 10000.0 nM -
CHEMBL2332859 IC50 > 10000.0 nM -