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Assay Detail

CHEMBL2344977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activity at BCRP (unknown origin) expressed in MDCK cells using rhodamine 123 as substrate incubated for 30 mins prior to substrate addition measured after 30 mins by fluorometric analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Potent and selective tariquidar bioisosters as potential PET radiotracers for imaging P-gp.
Contino M, Zinzi L, Perrone MG, Leopoldo M, Berardi F, Perrone R, Colabufo NA.
Bioorg Med Chem Lett (2013) 23 : 1370 -1374
PubMed 23374872 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 5.81 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL348475 TARIQUIDAR 3.0 1 8.00
CHEMBL2335007 1 7.75
CHEMBL2335006 1 6.30
CHEMBL2335002 1 6.00
CHEMBL2335008 1 5.00
CHEMBL260506 1 4.82
CHEMBL2335003 1 4.52
CHEMBL260599 1 4.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL348475 TARIQUIDAR EC50 = 10.0 nM 8.00
CHEMBL2335007 EC50 = 18.0 nM 7.75
CHEMBL2335006 EC50 = 500.0 nM 6.30
CHEMBL2335002 EC50 = 1000.0 nM 6.00
CHEMBL2335008 EC50 = 10000.0 nM 5.00
CHEMBL260506 EC50 = 15000.0 nM 4.82
CHEMBL2335003 EC50 = 30000.0 nM 4.52
CHEMBL260599 EC50 = 90000.0 nM 4.05