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Assay Detail

CHEMBL2345166

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant ADAMTS5 using bovine nasal cartilage aggrecan as substrate assessed as inhibition of 1772-AGEG neuropeptide formation incubated for 2 hrs prior to substrate addition measured after 2 hrs by Western blot analysis
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Nasal cartilage
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Arylsulfonamide inhibitors of aggrecanases as potential therapeutic agents for osteoarthritis: synthesis and biological evaluation.
Nuti E, Santamaria S, Casalini F, Yamamoto K, Marinelli L, La Pietra V, Novellino E, Orlandini E, Nencetti S, Marini AM, Salerno S, Taliani S, Da Settimo F, Nagase H, Rossello A.
Eur J Med Chem (2013) 62 : 379 -394
PubMed 23376997 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 5.96 7.11
Inhibition 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2337698 2 7.11
CHEMBL198778 2 6.08
CHEMBL2337691 2 4.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2337698 Ki = 77.0 nM 7.11
CHEMBL198778 Ki = 840.0 nM 6.08
CHEMBL2337691 Ki = 21000.0 nM 4.68
CHEMBL2337698 Inhibition - % -
CHEMBL198778 Inhibition - % -
CHEMBL2337691 Inhibition - % -