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Assay Detail

CHEMBL2351584

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human beta2 adrenoceptor expressed in CHOK1 cells assessed as inhibition of cimaterol-induced [3H]cAMP accumulation incubated for 15 mins prior to cimaterol induction measured after 5 hrs
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL210)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and in vitro and in vivo characterization of highly β1-selective β-adrenoceptor partial agonists.
Mistry SN, Baker JG, Fischer PM, Hill SJ, Gardiner SM, Kellam B.
J Med Chem (2013) 56 : 3852 -3865
PubMed 23614528 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 10 6.00 7.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3392321 1 7.25
CHEMBL75753 XAMOTEROL 2.0 1 6.18
CHEMBL2348201 1 6.10
CHEMBL2348218 1 6.05
CHEMBL280822 CGP-20712A 1 5.98
CHEMBL2348211 1 5.92
CHEMBL2348219 1 5.84
CHEMBL207802 LK-204545 1 5.54
CHEMBL2348220 1 5.15
CHEMBL2348217 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3392321 Kd = 56.23 nM 7.25
CHEMBL75753 XAMOTEROL Kd = 660.69 nM 6.18
CHEMBL2348201 Kd = 794.33 nM 6.10
CHEMBL2348218 Kd = 891.25 nM 6.05
CHEMBL280822 CGP-20712A Kd = 1047.13 nM 5.98
CHEMBL2348211 Kd = 1202.26 nM 5.92
CHEMBL2348219 Kd = 1445.44 nM 5.84
CHEMBL207802 LK-204545 Kd = 2884.03 nM 5.54
CHEMBL2348220 Kd = 7079.46 nM 5.15
CHEMBL2348217 Kd > 10000.0 nM -