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Assay Detail

CHEMBL2352544

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of diphenolase activity of mushroom tyrosinase assessed as decrease in L-DOPA chrome formation using L-DOPA as substrate preincubated with enzyme for 10 mins prior to substrate addition measured for 10 mins by spectrophotometric assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Agaricus bisporus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosinase (CHEMBL3318)
Type SINGLE PROTEIN
Organism Agaricus bisporus

Publication

Synthesis of novel azo compounds containing 5(4H)-oxazolone ring as potent tyrosinase inhibitors.
Hamidian H, Tagizadeh R, Fozooni S, Abbasalipour V, Taheri A, Namjou M.
Bioorg Med Chem (2013) 21 : 2088 -2092
PubMed 23411395 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.43 5.84

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2348690 1 5.84
CHEMBL2348689 1 5.70
CHEMBL2348688 1 5.52
CHEMBL245416 L-MIMOSINE 1 5.43
CHEMBL2348687 1 5.41
CHEMBL2348686 1 5.40
CHEMBL2348685 1 5.36
CHEMBL287556 KOJIC ACID 1 4.78

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2348690 IC50 = 1440.0 nM 5.84
CHEMBL2348689 IC50 = 2010.0 nM 5.70
CHEMBL2348688 IC50 = 3040.0 nM 5.52
CHEMBL245416 L-MIMOSINE IC50 = 3680.0 nM 5.43
CHEMBL2348687 IC50 = 3910.0 nM 5.41
CHEMBL2348686 IC50 = 3980.0 nM 5.40
CHEMBL2348685 IC50 = 4330.0 nM 5.36
CHEMBL287556 KOJIC ACID IC50 = 16670.0 nM 4.78