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Assay Detail

CHEMBL2379605

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of AKT phosphorylation at Ser 473 in human BT20 cells
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BT-20
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RAC-alpha serine/threonine-protein kinase (CHEMBL4282)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design, SAR analysis and antitumor activity of PI3K/mTOR dual inhibitors from 4-methylpyridopyrimidinone series.
Cheng H, Hoffman JE, Le PT, Pairish M, Kania R, Farrell W, Bagrodia S, Yuan J, Sun S, Zhang E, Xiang C, Dalvie D, Rahavendran SV.
Bioorg Med Chem Lett (2013) 23 : 2787 -2792
PubMed 23506825 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.25 8.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2375968 1 8.28
CHEMBL2375957 1 8.23
CHEMBL2375963 1 8.09
CHEMBL2376092 1 7.95
CHEMBL1234354 PF-04691502 2.0 1 7.89
CHEMBL2375958 1 7.71
CHEMBL2376095 1 7.67
CHEMBL2375365 1 7.66
CHEMBL2375964 1 7.63
CHEMBL2375965 1 7.62
CHEMBL2375967 1 7.59
CHEMBL2376093 1 7.53
CHEMBL2375956 1 7.29
CHEMBL2375953 1 7.25
CHEMBL2375961 1 7.08
CHEMBL2375969 1 6.98
CHEMBL2375959 1 6.94
CHEMBL2375962 1 6.83
CHEMBL2375955 1 6.59
CHEMBL2375966 1 6.54
CHEMBL2375970 1 6.51
CHEMBL2375954 1 6.42
CHEMBL2375960 1 6.00
CHEMBL2376096 1 5.68
CHEMBL2376094 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2375968 IC50 = 5.24 nM 8.28
CHEMBL2375957 IC50 = 5.82 nM 8.23
CHEMBL2375963 IC50 = 8.22 nM 8.09
CHEMBL2376092 IC50 = 11.2 nM 7.95
CHEMBL1234354 PF-04691502 IC50 = 13.0 nM 7.89
CHEMBL2375958 IC50 = 19.3 nM 7.71
CHEMBL2376095 IC50 = 21.6 nM 7.67
CHEMBL2375365 IC50 = 22.0 nM 7.66
CHEMBL2375964 IC50 = 23.3 nM 7.63
CHEMBL2375965 IC50 = 23.8 nM 7.62
CHEMBL2375967 IC50 = 25.9 nM 7.59
CHEMBL2376093 IC50 = 29.4 nM 7.53
CHEMBL2375956 IC50 = 51.9 nM 7.29
CHEMBL2375953 IC50 = 56.8 nM 7.25
CHEMBL2375961 IC50 = 82.9 nM 7.08
CHEMBL2375969 IC50 = 104.0 nM 6.98
CHEMBL2375959 IC50 = 116.0 nM 6.94
CHEMBL2375962 IC50 = 148.0 nM 6.83
CHEMBL2375955 IC50 = 256.0 nM 6.59
CHEMBL2375966 IC50 = 288.0 nM 6.54
CHEMBL2375970 IC50 = 310.0 nM 6.51
CHEMBL2375954 IC50 = 376.0 nM 6.42
CHEMBL2375960 IC50 = 988.0 nM 6.00
CHEMBL2376096 IC50 = 2070.0 nM 5.68
CHEMBL2376094 IC50 - -