Assay Detail
Binding
CHEMBL2383458
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of rifamycin-resistant Escherichia coli RNA polymerase D516V mutant after 10 mins by rolling circle transcription assay in presence of DNA nanocircle template NC45
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
DNA-directed RNA polymerase subunit beta (CHEMBL4296294) ↗| Type | SINGLE PROTEIN |
| Organism | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
Publication
X-ray crystal structures of the Escherichia coli RNA polymerase in complex with benzoxazinorifamycins.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 4.85 | 5.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2046886 | — | — | 1 | 5.70 |
| CHEMBL2046885 | — | — | 1 | 4.46 |
| CHEMBL2046887 | — | — | 1 | 4.39 |
| CHEMBL236297 | RIFALAZIL | 3.0 | 1 | - |
| CHEMBL374478 | RIFAMPIN | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2046886 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL2046885 | — | IC50 | = | 35000.0 | nM | 4.46 |
| CHEMBL2046887 | — | IC50 | = | 41000.0 | nM | 4.39 |
| CHEMBL236297 | RIFALAZIL | IC50 | = | 204000.0 | nM | - |
| CHEMBL374478 | RIFAMPIN | IC50 | = | 233000.0 | nM | - |