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Assay Detail

CHEMBL2388198

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Binding
Inhibition of mouse GAT3 expressed in HEK cells assessed as inhibition of [3H]-GABA uptake after 25 mins by liquid scintillation counting analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

GABA transporter 4 (CHEMBL3699)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and evaluation of N-substituted nipecotic acid derivatives with an unsymmetrical bis-aromatic residue attached to a vinyl ether spacer as potential GABA uptake inhibitors.
Quandt G, Höfner G, Wanner KT.
Bioorg Med Chem (2013) 21 : 3363 -3378
PubMed 23598250 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.60 4.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL96 GAMMA-AMINOBUTYRIC ACID 1.0 1 4.94
CHEMBL2387671 1 4.71
CHEMBL277498 NIPECOTIC ACID 1 4.71
CHEMBL2387675 1 4.70
CHEMBL2387677 1 4.55
CHEMBL2387669 1 4.46
CHEMBL2387666 1 4.43
CHEMBL2387673 1 4.30
CHEMBL2387662 1 -
CHEMBL2387658 1 -
CHEMBL2387678 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL96 GAMMA-AMINOBUTYRIC ACID IC50 = 11481.54 nM 4.94
CHEMBL2387671 IC50 = 19498.45 nM 4.71
CHEMBL277498 NIPECOTIC ACID IC50 = 19498.45 nM 4.71
CHEMBL2387675 IC50 = 19952.62 nM 4.70
CHEMBL2387677 IC50 = 28183.83 nM 4.55
CHEMBL2387669 IC50 = 34673.69 nM 4.46
CHEMBL2387666 IC50 = 37153.52 nM 4.43
CHEMBL2387673 IC50 = 50118.72 nM 4.30
CHEMBL2387662 IC50 = 338844.16 nM -
CHEMBL2387658 IC50 = 371535.23 nM -
CHEMBL2387678 IC50 = 194984.46 nM -