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Assay Detail

CHEMBL2389438

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TYK2 (unknown origin) assessed as inhibition of STAT4 phosphorylation by cell-based assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Non-receptor tyrosine-protein kinase TYK2 (CHEMBL3553)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Lead optimization of a 4-aminopyridine benzamide scaffold to identify potent, selective, and orally bioavailable TYK2 inhibitors.
Liang J, van Abbema A, Balazs M, Barrett K, Berezhkovsky L, Blair W, Chang C, Delarosa D, DeVoss J, Driscoll J, Eigenbrot C, Ghilardi N, Gibbons P, Halladay J, Johnson A, Kohli PB, Lai Y, Liu Y, Lyssikatos J, Mantik P, Menghrajani K, Murray J, Peng I, Sambrone A, Shia S, Shin Y, Smith J, Sohn S, Tsui V, Ultsch M, Wu LC, Xiao Y, Yang W, Young J, Zhang B, Zhu BY, Magnuson S.
J Med Chem (2013) 56 : 4521 -4536
PubMed 23668484 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 6.66 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1789941 RUXOLITINIB 4.0 1 8.00
CHEMBL2387221 1 6.84
CHEMBL2387124 1 6.80
CHEMBL2387127 1 6.71
CHEMBL2387126 1 6.70
CHEMBL2387222 1 6.68
CHEMBL2387223 1 6.65
CHEMBL2387225 1 6.61
CHEMBL2387119 1 6.56
CHEMBL2387224 1 6.42
CHEMBL2387110 1 6.41
CHEMBL2387112 1 6.36
CHEMBL2387118 1 6.34
CHEMBL221959 TOFACITINIB 4.0 1 6.20
CHEMBL2387219 1 -
CHEMBL2387218 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1789941 RUXOLITINIB EC50 = 10.0 nM 8.00
CHEMBL2387221 EC50 = 145.0 nM 6.84
CHEMBL2387124 EC50 = 157.0 nM 6.80
CHEMBL2387127 EC50 = 193.0 nM 6.71
CHEMBL2387126 EC50 = 198.0 nM 6.70
CHEMBL2387222 EC50 = 211.0 nM 6.68
CHEMBL2387223 EC50 = 224.0 nM 6.65
CHEMBL2387225 EC50 = 248.0 nM 6.61
CHEMBL2387119 EC50 = 273.0 nM 6.56
CHEMBL2387224 EC50 = 380.0 nM 6.42
CHEMBL2387110 EC50 = 393.0 nM 6.41
CHEMBL2387112 EC50 = 436.0 nM 6.36
CHEMBL2387118 EC50 = 452.0 nM 6.34
CHEMBL221959 TOFACITINIB EC50 = 625.0 nM 6.20
CHEMBL2387219 EC50 > 5000.0 nM -
CHEMBL2387218 EC50 > 5000.0 nM -