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Assay Detail

CHEMBL2390132

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant PDE4B (152 to 564 amino acids) using cAMP as substrate after 30 mins by plate reader analysis
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3',5'-cyclic-AMP phosphodiesterase 4B (CHEMBL275)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of the fused bicyclic 4-amino-2-phenylpyrimidine derivatives as novel and potent PDE4 inhibitors.
Goto T, Shiina A, Yoshino T, Mizukami K, Hirahara K, Suzuki O, Sogawa Y, Takahashi T, Mikkaichi T, Nakao N, Takahashi M, Hasegawa M, Sasaki S.
Bioorg Med Chem Lett (2013) 23 : 3325 -3328
PubMed 23602400 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 6.99 9.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 9.31
CHEMBL2385759 1 8.33
CHEMBL2385757 1 8.18
CHEMBL2385755 1 8.12
CHEMBL2385758 1 7.96
CHEMBL2385753 1 7.80
CHEMBL2385756 1 7.66
CHEMBL2385749 1 7.60
CHEMBL2385754 1 7.57
CHEMBL2385750 1 7.55
CHEMBL2385559 1 7.54
CHEMBL2385573 1 7.54
CHEMBL2385761 1 7.48
CHEMBL2385760 1 7.37
CHEMBL2385563 1 6.85
CHEMBL2385558 1 6.82
CHEMBL2385560 1 6.72
CHEMBL2385572 1 6.64
CHEMBL2385751 1 6.55
CHEMBL2385567 1 6.37
CHEMBL2385570 1 6.32
CHEMBL2385566 1 6.21
CHEMBL2385565 1 6.10
CHEMBL2385569 1 5.66
CHEMBL2385564 1 5.60
CHEMBL2385562 1 5.52
CHEMBL2385561 1 5.30
CHEMBL2385752 1 5.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST IC50 = 0.49 nM 9.31
CHEMBL2385759 IC50 = 4.7 nM 8.33
CHEMBL2385757 IC50 = 6.6 nM 8.18
CHEMBL2385755 IC50 = 7.5 nM 8.12
CHEMBL2385758 IC50 = 11.0 nM 7.96
CHEMBL2385753 IC50 = 16.0 nM 7.80
CHEMBL2385756 IC50 = 22.0 nM 7.66
CHEMBL2385749 IC50 = 25.0 nM 7.60
CHEMBL2385754 IC50 = 27.0 nM 7.57
CHEMBL2385750 IC50 = 28.0 nM 7.55
CHEMBL2385559 IC50 = 29.0 nM 7.54
CHEMBL2385573 IC50 = 29.0 nM 7.54
CHEMBL2385761 IC50 = 33.0 nM 7.48
CHEMBL2385760 IC50 = 43.0 nM 7.37
CHEMBL2385563 IC50 = 140.0 nM 6.85
CHEMBL2385558 IC50 = 150.0 nM 6.82
CHEMBL2385560 IC50 = 190.0 nM 6.72
CHEMBL2385572 IC50 = 230.0 nM 6.64
CHEMBL2385751 IC50 = 280.0 nM 6.55
CHEMBL2385567 IC50 = 430.0 nM 6.37
CHEMBL2385570 IC50 = 480.0 nM 6.32
CHEMBL2385566 IC50 = 610.0 nM 6.21
CHEMBL2385565 IC50 = 800.0 nM 6.10
CHEMBL2385569 IC50 = 2200.0 nM 5.66
CHEMBL2385564 IC50 = 2500.0 nM 5.60
CHEMBL2385562 IC50 = 3000.0 nM 5.52
CHEMBL2385561 IC50 = 5000.0 nM 5.30
CHEMBL2385752 IC50 = 7900.0 nM 5.10