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Assay Detail

CHEMBL2390171

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at FXR (unknown origin) expressed in human HepG2 cells co-expressing RXR assessed as inhibition of CDCA-induced transactivation after 18 hrs by luciferase reporter gene assay
3
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bile acid receptor (CHEMBL2047)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Binding mechanism of the farnesoid X receptor marine antagonist suvanine reveals a strategy to forestall drug modulation on nuclear receptors. Design, synthesis, and biological evaluation of novel ligands.
Di Leva FS, Festa C, D'Amore C, De Marino S, Renga B, D'Auria MV, Novellino E, Limongelli V, Zampella A, Fiorucci S.
J Med Chem (2013) 56 : 4701 -4717
PubMed 23656455 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 4.61 4.62
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1818099 SUVANINE'S SODIUM SALT 2 4.62
CHEMBL2386485 1 4.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1818099 SUVANINE'S SODIUM SALT EC50 = 24000.0 nM 4.62
CHEMBL2386485 EC50 = 25000.0 nM 4.60
CHEMBL1818099 SUVANINE'S SODIUM SALT Inhibition - % -