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Assay Detail

CHEMBL2390366

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK3 (unknown origin) using Leu-Pro-Leu-Asp-Lys-Asp-Tyr-Tyr-Val-Val-Arg as substrate after 30 mins in presence of ATP
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK3 (CHEMBL2148)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of C-2 hydroxyethyl imidazopyrrolopyridines as potent JAK1 inhibitors with favorable physicochemical properties and high selectivity over JAK2.
Zak M, Hurley CA, Ward SI, Bergeron P, Barrett K, Balazs M, Blair WS, Bull R, Chakravarty P, Chang C, Crackett P, Deshmukh G, DeVoss J, Dragovich PS, Eigenbrot C, Ellwood C, Gaines S, Ghilardi N, Gibbons P, Gradl S, Gribling P, Hamman C, Harstad E, Hewitt P, Johnson A, Johnson T, Kenny JR, Koehler MF, Bir Kohli P, Labadie S, Lee WP, Liao J, Liimatta M, Mendonca R, Narukulla R, Pulk R, Reeve A, Savage S, Shia S, Steffek M, Ubhayakar S, van Abbema A, Aliagas I, Avitabile-Woo B, Xiao Y, Yang J, Kulagowski JJ.
J Med Chem (2013) 56 : 4764 -4785
PubMed 23659214 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 7.66 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL221959 TOFACITINIB 4.0 1 9.40
CHEMBL2386635 1 8.23
CHEMBL2386636 1 8.11
CHEMBL2385096 1 7.28
CHEMBL2386629 1 7.12
CHEMBL2386653 1 6.96
CHEMBL2386633 1 6.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL221959 TOFACITINIB Ki = 0.4 nM 9.40
CHEMBL2386635 Ki = 5.9 nM 8.23
CHEMBL2386636 Ki = 7.8 nM 8.11
CHEMBL2385096 Ki = 53.0 nM 7.28
CHEMBL2386629 Ki = 76.0 nM 7.12
CHEMBL2386653 Ki = 110.0 nM 6.96
CHEMBL2386633 Ki = 280.0 nM 6.55