Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL2390546

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK1 in human TF1 cells assessed as inhibition of IL6-induced STAT3 phosphorylation incubated for 20 mins followed by IL6 challenge for 30 mins in presence of whole blood
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Cell Type TF-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of C-2 hydroxyethyl imidazopyrrolopyridines as potent JAK1 inhibitors with favorable physicochemical properties and high selectivity over JAK2.
Zak M, Hurley CA, Ward SI, Bergeron P, Barrett K, Balazs M, Blair WS, Bull R, Chakravarty P, Chang C, Crackett P, Deshmukh G, DeVoss J, Dragovich PS, Eigenbrot C, Ellwood C, Gaines S, Ghilardi N, Gibbons P, Gradl S, Gribling P, Hamman C, Harstad E, Hewitt P, Johnson A, Johnson T, Kenny JR, Koehler MF, Bir Kohli P, Labadie S, Lee WP, Liao J, Liimatta M, Mendonca R, Narukulla R, Pulk R, Reeve A, Savage S, Shia S, Steffek M, Ubhayakar S, van Abbema A, Aliagas I, Avitabile-Woo B, Xiao Y, Yang J, Kulagowski JJ.
J Med Chem (2013) 56 : 4764 -4785
PubMed 23659214 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 6.77 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2386635 1 7.55
CHEMBL2386629 1 7.54
CHEMBL2386636 1 7.48
CHEMBL221959 TOFACITINIB 4.0 1 7.37
CHEMBL2385096 1 7.29
CHEMBL2386630 1 7.21
CHEMBL2386634 1 7.11
CHEMBL2386632 1 7.09
CHEMBL2386633 1 6.89
CHEMBL2386627 1 6.85
CHEMBL2386653 1 6.75
CHEMBL2386645 1 6.66
CHEMBL2386628 1 6.55
CHEMBL2386647 1 6.50
CHEMBL2386637 1 6.50
CHEMBL2386648 1 6.48
CHEMBL2386640 1 6.48
CHEMBL2386625 1 6.14
CHEMBL2386646 1 6.09
CHEMBL2386638 1 5.82
CHEMBL2386643 1 5.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2386635 EC50 = 28.0 nM 7.55
CHEMBL2386629 EC50 = 29.0 nM 7.54
CHEMBL2386636 EC50 = 33.0 nM 7.48
CHEMBL221959 TOFACITINIB EC50 = 43.0 nM 7.37
CHEMBL2385096 EC50 = 51.0 nM 7.29
CHEMBL2386630 EC50 = 62.0 nM 7.21
CHEMBL2386634 EC50 = 78.0 nM 7.11
CHEMBL2386632 EC50 = 82.0 nM 7.09
CHEMBL2386633 EC50 = 130.0 nM 6.89
CHEMBL2386627 EC50 = 140.0 nM 6.85
CHEMBL2386653 EC50 = 180.0 nM 6.75
CHEMBL2386645 EC50 = 220.0 nM 6.66
CHEMBL2386628 EC50 = 280.0 nM 6.55
CHEMBL2386647 EC50 = 320.0 nM 6.50
CHEMBL2386637 EC50 = 313.0 nM 6.50
CHEMBL2386648 EC50 = 330.0 nM 6.48
CHEMBL2386640 EC50 = 330.0 nM 6.48
CHEMBL2386625 EC50 = 730.0 nM 6.14
CHEMBL2386646 EC50 = 820.0 nM 6.09
CHEMBL2386638 EC50 = 1500.0 nM 5.82
CHEMBL2386643 EC50 = 1600.0 nM 5.80