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Assay Detail

CHEMBL2390624

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at CRTh2 receptor (unknown origin) overexpressed in CHOK1 cell membranes assessed as inhibition of [35S]-GTPgammaS binding preincubated for 1 hr followed by 50 nM PGD2 and 0.1 nM [35S]-GTPgammaS addition measured after 2 hrs by liquid scintillation counting analysis
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin D2 receptor 2 (CHEMBL5071)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-(1H-Pyrazol-4-yl)acetic acids as CRTh2 antagonists.
Andrés M, Bravo M, Buil MA, Calbet M, Castro J, Domènech T, Eichhorn P, Ferrer M, Gómez E, Lehner MD, Moreno I, Roberts RS, Sevilla S.
Bioorg Med Chem Lett (2013) 23 : 3349 -3353
PubMed 23601708 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 6.46 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2385113 1 8.40
CHEMBL2387692 1 8.15
CHEMBL2385124 1 7.80
CHEMBL2385112 1 7.62
CHEMBL2387698 1 7.44
CHEMBL2385115 1 7.34
CHEMBL2387700 1 7.18
CHEMBL2385125 1 7.09
CHEMBL2387699 1 7.05
CHEMBL2387694 1 7.00
CHEMBL560993 TIMAPIPRANT 3.0 1 6.89
CHEMBL2385114 1 6.80
CHEMBL2387691 1 6.64
CHEMBL2385117 1 6.54
CHEMBL2385118 1 6.51
CHEMBL2387693 1 6.31
CHEMBL2385109 1 6.07
CHEMBL2387688 1 6.06
CHEMBL2385120 1 5.96
CHEMBL2387695 1 5.85
CHEMBL2385131 1 5.80
CHEMBL2385122 1 5.80
CHEMBL2385116 1 5.72
CHEMBL2387697 1 5.70
CHEMBL2385110 1 5.54
CHEMBL2385108 1 5.30
CHEMBL2387689 1 5.30
CHEMBL2387696 1 5.30
CHEMBL2387690 1 5.30
CHEMBL2385121 1 5.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2385113 IC50 = 4.0 nM 8.40
CHEMBL2387692 IC50 = 7.0 nM 8.15
CHEMBL2385124 IC50 = 16.0 nM 7.80
CHEMBL2385112 IC50 = 24.0 nM 7.62
CHEMBL2387698 IC50 = 36.0 nM 7.44
CHEMBL2385115 IC50 = 46.0 nM 7.34
CHEMBL2387700 IC50 = 66.0 nM 7.18
CHEMBL2385125 IC50 = 81.0 nM 7.09
CHEMBL2387699 IC50 = 89.0 nM 7.05
CHEMBL2387694 IC50 = 100.0 nM 7.00
CHEMBL560993 TIMAPIPRANT IC50 = 130.0 nM 6.89
CHEMBL2385114 IC50 = 160.0 nM 6.80
CHEMBL2387691 IC50 = 230.0 nM 6.64
CHEMBL2385117 IC50 = 290.0 nM 6.54
CHEMBL2385118 IC50 = 310.0 nM 6.51
CHEMBL2387693 IC50 = 490.0 nM 6.31
CHEMBL2385109 IC50 = 850.0 nM 6.07
CHEMBL2387688 IC50 = 870.0 nM 6.06
CHEMBL2385120 IC50 = 1100.0 nM 5.96
CHEMBL2387695 IC50 = 1400.0 nM 5.85
CHEMBL2385131 IC50 = 1600.0 nM 5.80
CHEMBL2385122 IC50 = 1600.0 nM 5.80
CHEMBL2385116 IC50 = 1900.0 nM 5.72
CHEMBL2387697 IC50 = 2000.0 nM 5.70
CHEMBL2385110 IC50 = 2900.0 nM 5.54
CHEMBL2385108 IC50 = 5000.0 nM 5.30
CHEMBL2387689 IC50 = 5000.0 nM 5.30
CHEMBL2387696 IC50 = 5000.0 nM 5.30
CHEMBL2387690 IC50 = 5000.0 nM 5.30
CHEMBL2385121 IC50 = 5000.0 nM 5.30
CHEMBL2385123 IC50 - -
CHEMBL2385111 IC50 - -
CHEMBL2387687 IC50 > 5000.0 nM -
CHEMBL2385119 IC50 - -