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Assay Detail

CHEMBL2395566

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human GPR35b expressed in CHO cells assessed as induction of beta-arrestin recruitment after 90 mins by beta-galactosidase reporter gene assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 35 (CHEMBL1293267)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

8-Benzamidochromen-4-one-2-carboxylic acids: potent and selective agonists for the orphan G protein-coupled receptor GPR35.
Funke M, Thimm D, Schiedel AC, Müller CE.
J Med Chem (2013) 56 : 5182 -5197
PubMed 23713606 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 6.90 7.83

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2392179 1 7.83
CHEMBL2392160 1 7.69
CHEMBL2392174 1 7.66
CHEMBL2392172 1 7.57
CHEMBL2392167 1 7.54
CHEMBL2392171 1 7.46
CHEMBL2392170 1 7.39
CHEMBL2392161 1 6.97
CHEMBL2392178 1 6.89
CHEMBL2392162 1 6.84
CHEMBL2392157 1 6.33
CHEMBL2392177 1 6.20
CHEMBL2392158 1 6.17
CHEMBL2392169 1 6.16
CHEMBL2392159 1 6.06
CHEMBL28079 ZAPRINAST 2.0 1 5.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2392179 EC50 = 14.7 nM 7.83
CHEMBL2392160 EC50 = 20.5 nM 7.69
CHEMBL2392174 EC50 = 22.1 nM 7.66
CHEMBL2392172 EC50 = 27.2 nM 7.57
CHEMBL2392167 EC50 = 28.6 nM 7.54
CHEMBL2392171 EC50 = 34.6 nM 7.46
CHEMBL2392170 EC50 = 40.5 nM 7.39
CHEMBL2392161 EC50 = 106.0 nM 6.97
CHEMBL2392178 EC50 = 128.0 nM 6.89
CHEMBL2392162 EC50 = 143.0 nM 6.84
CHEMBL2392157 EC50 = 472.0 nM 6.33
CHEMBL2392177 EC50 = 628.0 nM 6.20
CHEMBL2392158 EC50 = 678.0 nM 6.17
CHEMBL2392169 EC50 = 699.0 nM 6.16
CHEMBL2392159 EC50 = 873.0 nM 6.06
CHEMBL28079 ZAPRINAST EC50 = 2240.0 nM 5.65