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Assay Detail

CHEMBL2395572

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at mouse GPR35 expressed in CHO cells assessed as induction of beta-arrestin recruitment after 90 mins by beta-galactosidase reporter gene assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 35 (CHEMBL2390813)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

8-Benzamidochromen-4-one-2-carboxylic acids: potent and selective agonists for the orphan G protein-coupled receptor GPR35.
Funke M, Thimm D, Schiedel AC, Müller CE.
J Med Chem (2013) 56 : 5182 -5197
PubMed 23713606 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 5.35 6.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL28079 ZAPRINAST 2.0 1 6.01
CHEMBL2392151 1 5.52
CHEMBL2392152 1 5.45
CHEMBL74 CROMOLYN SODIUM 4.0 1 5.32
CHEMBL2392183 1 5.30
CHEMBL2392176 1 5.30
CHEMBL2392157 1 5.30
CHEMBL2392177 1 5.19
CHEMBL2392141 1 4.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL28079 ZAPRINAST EC50 = 980.0 nM 6.01
CHEMBL2392151 EC50 = 2990.0 nM 5.52
CHEMBL2392152 EC50 = 3590.0 nM 5.45
CHEMBL74 CROMOLYN SODIUM EC50 = 4840.0 nM 5.32
CHEMBL2392183 EC50 = 4980.0 nM 5.30
CHEMBL2392176 EC50 = 4950.0 nM 5.30
CHEMBL2392157 EC50 = 5030.0 nM 5.30
CHEMBL2392177 EC50 = 6400.0 nM 5.19
CHEMBL2392141 EC50 = 17000.0 nM 4.77