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Assay Detail

CHEMBL2404670

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human AChE using acetylthiocholine iodide as substrate treated 5 mins before substrate addition measured up to 4 mins by Ellman's method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective inhibition of human acetylcholinesterase by xanthine derivatives: in vitro inhibition and molecular modeling investigations.
Mohamed T, Osman W, Tin G, Rao PP.
Bioorg Med Chem Lett (2013) 23 : 4336 -4341
PubMed 23791077 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.79 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502 DONEPEZIL 4.0 1 7.40
CHEMBL95 TACRINE 4.0 1 6.80
CHEMBL1079905 PROPENTOFYLLINE 2.0 1 5.19
CHEMBL628 PENTOXIFYLLINE 4.0 1 5.18
CHEMBL113 CAFFEINE 4.0 1 5.14
CHEMBL1424 XANTHINE 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502 DONEPEZIL IC50 = 40.0 nM 7.40
CHEMBL95 TACRINE IC50 = 160.0 nM 6.80
CHEMBL1079905 PROPENTOFYLLINE IC50 = 6400.0 nM 5.19
CHEMBL628 PENTOXIFYLLINE IC50 = 6600.0 nM 5.18
CHEMBL113 CAFFEINE IC50 = 7250.0 nM 5.14
CHEMBL1424 XANTHINE IC50 = 9000.0 nM 5.05