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Assay Detail

CHEMBL2405979

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human p38alpha expressed in Escherichia coli using MBP as substrate preincubated for 10 mins prior to substrate addition measured after 45 mins by microbeta scintillation counting analysis
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 14 (CHEMBL260)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The identification of novel p38α isoform selective kinase inhibitors having an unprecedented p38α binding mode.
Wrobleski ST, Lin S, Dhar TG, Dyckman AJ, Li T, Pitt S, Zhang R, Fan Y, Doweyko AM, Tokarski JS, Kish KF, Kiefer SE, Sack JS, Newitt JA, Witmer MR, McKinnon M, Barrish JC, Dodd JH, Schieven GL, Leftheris K.
Bioorg Med Chem Lett (2013) 23 : 4120 -4126
PubMed 23746475 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 8.17 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2401994 1 9.70
CHEMBL2401995 1 9.10
CHEMBL2401981 1 8.70
CHEMBL2402000 1 8.55
CHEMBL2401985 1 8.42
CHEMBL2401993 1 8.42
CHEMBL258202 1 8.40
CHEMBL2401979 1 8.40
CHEMBL2401988 1 8.40
CHEMBL2401987 1 8.39
CHEMBL2401989 1 8.39
CHEMBL2401983 1 8.32
CHEMBL2401990 1 8.30
CHEMBL2401991 1 8.27
CHEMBL2401978 1 8.15
CHEMBL2401999 1 8.11
CHEMBL2402001 1 8.11
CHEMBL2401984 1 8.10
CHEMBL2401992 1 8.07
CHEMBL2401982 1 8.05
CHEMBL2401998 1 7.96
CHEMBL2401997 1 7.89
CHEMBL2401986 1 7.85
CHEMBL2402005 1 7.64
CHEMBL2402002 1 7.50
CHEMBL2402004 1 7.47
CHEMBL2402003 1 7.46
CHEMBL2401996 1 7.39
CHEMBL2401980 1 7.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2401994 IC50 = 0.2 nM 9.70
CHEMBL2401995 IC50 = 0.8 nM 9.10
CHEMBL2401981 IC50 = 2.0 nM 8.70
CHEMBL2402000 IC50 = 2.8 nM 8.55
CHEMBL2401985 IC50 = 3.8 nM 8.42
CHEMBL2401993 IC50 = 3.8 nM 8.42
CHEMBL258202 IC50 = 4.0 nM 8.40
CHEMBL2401979 IC50 = 4.0 nM 8.40
CHEMBL2401988 IC50 = 4.0 nM 8.40
CHEMBL2401987 IC50 = 4.1 nM 8.39
CHEMBL2401989 IC50 = 4.1 nM 8.39
CHEMBL2401983 IC50 = 4.8 nM 8.32
CHEMBL2401990 IC50 = 5.0 nM 8.30
CHEMBL2401991 IC50 = 5.4 nM 8.27
CHEMBL2401978 IC50 = 7.0 nM 8.15
CHEMBL2401999 IC50 = 7.7 nM 8.11
CHEMBL2402001 IC50 = 7.7 nM 8.11
CHEMBL2401984 IC50 = 8.0 nM 8.10
CHEMBL2401992 IC50 = 8.6 nM 8.07
CHEMBL2401982 IC50 = 8.9 nM 8.05
CHEMBL2401998 IC50 = 11.0 nM 7.96
CHEMBL2401997 IC50 = 13.0 nM 7.89
CHEMBL2401986 IC50 = 14.0 nM 7.85
CHEMBL2402005 IC50 = 23.0 nM 7.64
CHEMBL2402002 IC50 = 32.0 nM 7.50
CHEMBL2402004 IC50 = 34.0 nM 7.47
CHEMBL2402003 IC50 = 35.0 nM 7.46
CHEMBL2401996 IC50 = 41.0 nM 7.39
CHEMBL2401980 IC50 = 46.0 nM 7.34